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  1. Link

    pkc-zeta-antibody-ab227250.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC
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Anti-PKC zeta antibody (ab227250)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta antibody (ab227250)
  • Western blot - Anti-PKC zeta antibody (ab227250)

Key features and details

  • Rabbit polyclonal to PKC zeta
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant human PKC zeta protein (ab60848)

View more associated products

Overview

  • Product name

    Anti-PKC zeta antibody
    See all PKC zeta primary antibodies
  • Description

    Rabbit polyclonal to PKC zeta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig, Zebrafish
  • Immunogen

    Recombinant fragment within Human PKC zeta (internal sequence). The exact sequence is proprietary.
    Database link: Q05513

  • Positive control

    • IHC-P: SW480 xenograft tissue. WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • SK N SH whole cell lysate (ab3956)
  • Recombinant Protein

    • Recombinant human PKC zeta protein (ab60848)
  • Related Products

    • Recombinant human PKC zeta protein (ab60848)

Applications

Our Abpromise guarantee covers the use of ab227250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
  • Tissue specificity

    Expressed in brain, and to a lesser extent in lung, kidney and testis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 OPR domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • Domain

    The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
    The C1 domain does not bind the diacylglycerol (DAG).
  • Post-translational
    modifications

    CDH5 is required for its phosphorylation at Thr-410.
  • Cellular localization

    Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Target information above from: UniProt accession Q05513 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 286877 Cow
    • Entrez Gene: 5590 Human
    • Entrez Gene: 18762 Mouse
    • Entrez Gene: 100533200 Pig
    • Entrez Gene: 100009538 Rabbit
    • Entrez Gene: 25522 Rat
    • Entrez Gene: 555737 Zebrafish
    • Omim: 176982 Human
    • SwissProt: Q05513 Human
    • SwissProt: Q02956 Mouse
    • SwissProt: O19111 Rabbit
    • SwissProt: P09217 Rat
    • SwissProt: Q4JG04 Zebrafish
    • Unigene: 496255 Human
    • Unigene: 28561 Mouse
    • Unigene: 1109 Rat
    see all
  • Alternative names

    • 14-3-3-zetaisoform antibody
    • AI098070 antibody
    • aPKCzeta antibody
    • C80388 antibody
    • EC 2.7.11.13 antibody
    • KPCZ_HUMAN antibody
    • nPKC zeta antibody
    • nPKC-zeta antibody
    • OTTHUMP00000001368 antibody
    • OTTHUMP00000044160 antibody
    • PKC 2 antibody
    • PKC ZETA antibody
    • PKC2 antibody
    • Pkcz antibody
    • PKCZETA antibody
    • PKM-zeta, included antibody
    • PRKCZ antibody
    • Protein kinase C zeta antibody
    • Protein kinase C zeta form antibody
    • Protein kinase C zeta type antibody
    • r14-3-3 antibody
    • R74924 antibody
    • zetaPKC antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta antibody (ab227250)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta antibody (ab227250)

    Paraffin-embedded SW480 xenograft tissue stained for PKC zeta using ab227250 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-PKC zeta antibody (ab227250)
    Western blot - Anti-PKC zeta antibody (ab227250)
    All lanes : Anti-PKC zeta antibody (ab227250) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
    Lane 3 : IMR32 whole cell extract
    Lane 4 : SK-N-AS whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 68 kDa



    7.5% SDS-PAGE gel.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab227250? Please let us know so that we can cite the reference in this datasheet.

    ab227250 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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