Overview

  • Product name
  • Description
    Rabbit polyclonal to PKC zeta
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, IHC-Fr, ICC, IPmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mammals
  • Immunogen

    Synthetic peptide:

    EYINPLLLSAEESV

    , corresponding to amino acids 579-592 of Rat PKC zeta.

  • Positive control
    • Rat brain and myeloid tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Predicted molecular weight: 71 kDa. (using horseradish peroxidase or alkaline phosphatase).
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC 1/250. Frozen. (using horseradish peroxidase or alkaline phosphatase).
IP 1/300. PubMed: 19028678

Target

  • Function
    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
  • Tissue specificity
    Expressed in brain, and to a lesser extent in lung, kidney and testis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 OPR domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • Domain
    The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
    The C1 domain does not bind the diacylglycerol (DAG).
  • Post-translational
    modifications
    CDH5 is required for its phosphorylation at Thr-410.
  • Cellular localization
    Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14-3-3-zetaisoform antibody
    • AI098070 antibody
    • aPKCzeta antibody
    • C80388 antibody
    • EC 2.7.11.13 antibody
    • KPCZ_HUMAN antibody
    • nPKC zeta antibody
    • nPKC-zeta antibody
    • OTTHUMP00000001368 antibody
    • OTTHUMP00000044160 antibody
    • PKC 2 antibody
    • PKC ZETA antibody
    • PKC2 antibody
    • Pkcz antibody
    • PKCZETA antibody
    • PKM-zeta, included antibody
    • PRKCZ antibody
    • Protein kinase C zeta antibody
    • Protein kinase C zeta form antibody
    • Protein kinase C zeta type antibody
    • r14-3-3 antibody
    • R74924 antibody
    • zetaPKC antibody
    see all

Images

  • Immunoprecipitation.
    Rat renal cortex from adult male Sprague-Dawley CD rats was isolated and suspended in CelLyticTM MT Mammalian Tissue Lysis/Extraction reagent containing complete protease inhibitor mixture. Tissues were then homogenized and centrifuged at 2,500 x g for 10 minutes at 4 °C. The supernatant was used for immunoprecipitation. To eliminate nonspecific binding, 1 ml of rat kidney lysate (3 mg of protein) was pre-cleared by incubation with 50µl of TrueBlotTM anti-rabbit Ig IP beads overnight at 4 °C. Cleared cell lysates were incubated with either 7µg of ab4137 or 1µg of polyclonal rabbit anti-rOAT3 for 2 hours at 4 °C. TrueBlot anti-rabbit Ig IP beads (30µl) was added and incubation was continued for 2 hours at 4 °C. Bound proteins were washed 7 times with increasing washing stringency IP buffers and eluted from beads by heating in 4 x NuPAGE LDS sample buffer for 10 minutes. The Invitrogen NuPAGE Novex Tris

References

This product has been referenced in:
  • Levy D  et al. Lysine methylation of the NF-?B subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-?B signaling. Nat Immunol 12:29-36 (2011). Read more (PubMed: 21131967) »
  • Barros SA  et al. Activation of protein kinase Czeta increases OAT1 (SLC22A6)- and OAT3 (SLC22A8)-mediated transport. J Biol Chem 284:2672-9 (2009). WB, IP ; Rat . Read more (PubMed: 19028678) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your inquiry. I have conducted a search for you and found the following antibodies: ab43176 - Rabbit polyclonal to PKC zeta ab4137 - Chicken polyclonal to ATP Synthase beta ab51157 - Rabbit polyclonal to PKC zeta ab59412 - Rabbit polyclonal to PKC zeta (phospho T560) ab62372 - Rabbit monoclonal [EP2037AY] to PKC zeta (phospho T560)  We have a number of antibodies in our catalog that might be of interest to you. I would like to direct you to the search facility on our website (www.abcam.com). Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in. I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.              

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Answer

Thank you for your patience and for providing details regarding your protocol. Ab4137 was characterized for use in Western blotting with rat brain and myeloid tissues. This antibody has not yet been tested for cross-reactivity with human samples. I would therefore recommend using rat brain or myeloid tissue as a positive control. The antibody is predicted to react with other mammals due to sequence homology, but this hasn't been tested so we cannot guarantee results. The originator of this antibody also mentioned that the calculated molecular weight is 67.7 kDa but it is common to find that the protein resolves closer to 76-80 kDa. I know that you mentioned seeing a band at approximately 50 kDa, but try the antibody again with a positive control (also, make sure that there are sufficient protease inhibitors in your sample buffer). I hope this helps, please contact us again if you need additional assistance.

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Answer

Thank you for your enquiry. Did you try increasing the concentration of the primary to 1:100? Please let me know if that improved your results. What specifically are the rat samples that you are using? Can you tell me as well, how did you store the antibody? Thanks again, and I look forward to hearing from you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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