Product nameAnti-PKC zeta antibody
See all PKC zeta primary antibodies
DescriptionRabbit polyclonal to PKC zeta
Tested applicationsSuitable for: WB, ELISA, IHC-Fr, ICC, IPmore details
Species reactivityReacts with: Rat
Predicted to work with: Mammals
- Rat brain and myeloid tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Our Abpromise guarantee covers the use of ab4137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Predicted molecular weight: 71 kDa. (using horseradish peroxidase or alkaline phosphatase).|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||1/250. Frozen. (using horseradish peroxidase or alkaline phosphatase).|
|IP||1/300. PubMed: 19028678|
FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
Tissue specificityExpressed in brain, and to a lesser extent in lung, kidney and testis.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
DomainThe OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
The C1 domain does not bind the diacylglycerol (DAG).
modificationsCDH5 is required for its phosphorylation at Thr-410.
Cellular localizationCytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
- Information by UniProt
- 14-3-3-zetaisoform antibody
- AI098070 antibody
- aPKCzeta antibody
Rat renal cortex from adult male Sprague-Dawley CD rats was isolated and suspended in CelLyticTM MT Mammalian Tissue Lysis/Extraction reagent containing complete protease inhibitor mixture. Tissues were then homogenized and centrifuged at 2,500 x g for 10 minutes at 4 °C. The supernatant was used for immunoprecipitation. To eliminate nonspecific binding, 1 ml of rat kidney lysate (3 mg of protein) was pre-cleared by incubation with 50µl of TrueBlotTM anti-rabbit Ig IP beads overnight at 4 °C. Cleared cell lysates were incubated with either 7µg of ab4137 or 1µg of polyclonal rabbit anti-rOAT3 for 2 hours at 4 °C. TrueBlot anti-rabbit Ig IP beads (30µl) was added and incubation was continued for 2 hours at 4 °C. Bound proteins were washed 7 times with increasing washing stringency IP buffers and eluted from beads by heating in 4 x NuPAGE LDS sample buffer for 10 minutes. The Invitrogen NuPAGE Novex Tris
This product has been referenced in:
- Levy D et al. Lysine methylation of the NF-?B subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-?B signaling. Nat Immunol 12:29-36 (2011). Read more (PubMed: 21131967) »
- Barros SA et al. Activation of protein kinase Czeta increases OAT1 (SLC22A6)- and OAT3 (SLC22A8)-mediated transport. J Biol Chem 284:2672-9 (2009). WB, IP ; Rat . Read more (PubMed: 19028678) »