Anti-PKC zeta antibody - C-terminal (ab226772)
Key features and details
- Rabbit polyclonal to PKC zeta - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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Overview
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Product name
Anti-PKC zeta antibody - C-terminal
See all PKC zeta primary antibodies -
Description
Rabbit polyclonal to PKC zeta - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig, Zebrafish -
Immunogen
Synthetic peptide within Human PKC zeta (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q05513 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 68 kDa.
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IP |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 68 kDa. |
IP
1/100 - 1/500. |
Target
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Function
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization. -
Tissue specificity
Expressed in brain, and to a lesser extent in lung, kidney and testis. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
Domain
The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
The C1 domain does not bind the diacylglycerol (DAG). -
Post-translational
modificationsCDH5 is required for its phosphorylation at Thr-410. -
Cellular localization
Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. - Information by UniProt
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Database links
- Entrez Gene: 286877 Cow
- Entrez Gene: 5590 Human
- Entrez Gene: 18762 Mouse
- Entrez Gene: 100533200 Pig
- Entrez Gene: 100009538 Rabbit
- Entrez Gene: 25522 Rat
- Entrez Gene: 555737 Zebrafish
- Omim: 176982 Human
see all -
Alternative names
- 14-3-3-zetaisoform antibody
- AI098070 antibody
- aPKCzeta antibody
see all
Images
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All lanes : Anti-PKC zeta antibody - C-terminal (ab226772) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa10% SDS-PAGE gel.
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PKC zeta was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg ab226772. Western blot was performed from the immunoprecipitate using ab226772. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in HEK-293T whole cell extract.
Lane 2: ab226772 IP in HEK-293T whole cell extract.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226772 has not yet been referenced specifically in any publications.