Anti-PKC zeta antibody [EP1490(2)] (ab108970)
Key features and details
- Rabbit monoclonal [EP1490(2)] to PKC zeta
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PKC zeta antibody [EP1490(2)]
See all PKC zeta primary antibodies -
Description
Rabbit monoclonal [EP1490(2)] to PKC zeta -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, HT-29 and fetal liver lysates
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1490(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 74 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 74 kDa. |
Target
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Function
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization. -
Tissue specificity
Expressed in brain, and to a lesser extent in lung, kidney and testis. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
Domain
The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
The C1 domain does not bind the diacylglycerol (DAG). -
Post-translational
modificationsCDH5 is required for its phosphorylation at Thr-410. -
Cellular localization
Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. - Information by UniProt
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Database links
- Entrez Gene: 5590 Human
- Entrez Gene: 18762 Mouse
- Entrez Gene: 25522 Rat
- Omim: 176982 Human
- SwissProt: Q05513 Human
- SwissProt: Q02956 Mouse
- SwissProt: P09217 Rat
- Unigene: 496255 Human
see all -
Alternative names
- 14-3-3-zetaisoform antibody
- AI098070 antibody
- aPKCzeta antibody
see all
Images
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All lanes : Anti-PKC zeta antibody [EP1490(2)] (ab108970) at 1/1000 dilution
Lane 1 : 293T lysate
Lane 2 : HT-29 lysate
Lane 3 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa -
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PKC zeta knockout HAP1 cell lysate (20 µg)
Lane 3: HT-29 cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108970 observed at 74 kDa. Red - loading control, ab8226, observed at 42 kDa.
ab108970 was shown to specifically react with PKC zeta when PKC zeta knockout samples were used. Wild-type and PKC zeta knockout samples were subjected to SDS-PAGE. ab108970 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
All lanes : Anti-PKC zeta antibody [EP1490(2)] (ab108970) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Rat lung tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 74 kDaLanes 1 - 3: Merged signal (red and green). Green - ab108970 observed at 74 kDa. Red - loading control, ab8245, observed at 37 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab108970 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab108970 has been referenced in 1 publication.
- Liu S et al. G3BP1 regulates breast cancer cell proliferation and metastasis by modulating PKCζ. Front Genet 13:1034889 (2022). PubMed: 36330442