Overview

  • Product name

    Anti-PKC zeta antibody [EP1490(2)] - BSA and Azide free
    See all PKC zeta primary antibodies
  • Description

    Rabbit monoclonal [EP1490(2)] to PKC zeta - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PKC zeta (UniProt Q05513).

  • Positive control

    • 293T, HT-29 and fetal liver lysates
  • General notes

    Ab225554 is the carrier-free version of ab108970. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EP1490(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 74 kDa.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
    • Tissue specificity

      Expressed in brain, and to a lesser extent in lung, kidney and testis.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 OPR domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • Domain

      The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
      The C1 domain does not bind the diacylglycerol (DAG).
    • Post-translational
      modifications

      CDH5 is required for its phosphorylation at Thr-410.
    • Cellular localization

      Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
    • Information by UniProt
    • Database links

    • Alternative names

      • 14-3-3-zetaisoform antibody
      • AI098070 antibody
      • aPKCzeta antibody
      • C80388 antibody
      • EC 2.7.11.13 antibody
      • KPCZ_HUMAN antibody
      • nPKC zeta antibody
      • nPKC-zeta antibody
      • OTTHUMP00000001368 antibody
      • OTTHUMP00000044160 antibody
      • PKC 2 antibody
      • PKC ZETA antibody
      • PKC2 antibody
      • Pkcz antibody
      • PKCZETA antibody
      • PKM-zeta, included antibody
      • PRKCZ antibody
      • Protein kinase C zeta antibody
      • Protein kinase C zeta form antibody
      • Protein kinase C zeta type antibody
      • r14-3-3 antibody
      • R74924 antibody
      • zetaPKC antibody
      see all

    Images

    • This WB data was generated using the same anti-PKC zeta antibody clone [EP1490(2)] in a different buffer formulation (cat# ab108970).

      Lanes 1 - 3: Merged signal (red and green). Green - ab108970 observed at 74 kDa. Red - loading control, ab8245, observed at 37 kDa.

       

      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab108970 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling PKC zeta with unpurified ab108970 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108970).

    • ab108970 staining PKC zeta in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody (1/400 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108970).

    References

    ab225554 has not yet been referenced specifically in any publications.

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