Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)


  • Product name
    Anti-PKC zeta (phospho T560) antibody [EP2037AY]
    See all PKC zeta primary antibodies
  • Description
    Rabbit monoclonal [EP2037AY] to PKC zeta (phospho T560)
  • Host species
  • Specificity
    ab62372 detects PKC zeta phosphorylated at Threonine 560.
  • Tested applications
    Suitable for: WB, IHC-P, Dot blotmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PKC zeta aa 550-650 (phospho T560). The exact sequence is proprietary.

  • Positive control
    • WB: HeLa cell lysates. IHC-P: Human brain tissue and human breast ductal carcinoma tissue.
  • General notes

    A trial size is available to purchase for this antibody.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab62372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/100.
Dot blot 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
    • Tissue specificity
      Expressed in brain, and to a lesser extent in lung, kidney and testis.
    • Sequence similarities
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 OPR domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • Domain
      The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
      The C1 domain does not bind the diacylglycerol (DAG).
    • Post-translational
      CDH5 is required for its phosphorylation at Thr-410.
    • Cellular localization
      Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
    • Information by UniProt
    • Database links
    • Alternative names
      • 14-3-3-zetaisoform antibody
      • AI098070 antibody
      • aPKCzeta antibody
      • C80388 antibody
      • EC antibody
      • KPCZ_HUMAN antibody
      • nPKC zeta antibody
      • nPKC-zeta antibody
      • OTTHUMP00000001368 antibody
      • OTTHUMP00000044160 antibody
      • PKC 2 antibody
      • PKC ZETA antibody
      • PKC2 antibody
      • Pkcz antibody
      • PKCZETA antibody
      • PKM-zeta, included antibody
      • PRKCZ antibody
      • Protein kinase C zeta antibody
      • Protein kinase C zeta form antibody
      • Protein kinase C zeta type antibody
      • r14-3-3 antibody
      • R74924 antibody
      • zetaPKC antibody
      see all


    • All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372) at 1/20000 dilution

      Lane 1 : HeLa cell lysate - treated with Calyculin A
      Lane 2 : HeLa cell lysate - treated with Calyculin A and lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 68 kDa

      Exposure time: 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

    • ab62372 at 1/100 dilution staining PKC zeta in human brain by Immunohistochemistry, Paraffin-embedded tissue.
    • Dot blot analysis of PKC zeta (pT560) peptide (Lane 1) and PKC zeta non-phospho peptide (Lane 2) labelling PKC zeta (pT560) with ab62372 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

      Lane 1 : Hela Cell Lysate (untreated with Calyculin A)
      Lane 2 : Hela Cell Lysate (Treated with Calyculin A)

      Predicted band size: 68 kDa

    • ab62372 at 1/100 dilution staining PKC zeta in human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.


    This product has been referenced in:
    • Chibly AM  et al. aPKC?-dependent Repression of Yap is Necessary for Functional Restoration of Irradiated Salivary Glands with IGF-1. Sci Rep 8:6347 (2018). Read more (PubMed: 29679075) »
    • Lahey KA  et al. Signaling pathways induced by serine proteases to increase intestinal epithelial barrier function. PLoS One 12:e0180259 (2017). Read more (PubMed: 28671992) »

    See all 12 Publications for this product

    Customer reviews and Q&As

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