Overview

  • Product name

  • Description

    Rabbit polyclonal to PKD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PKD2 aa 320-400. The exact sequence is proprietary. NP_057541.2
    Database link: Q9BZL6

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
  • General notes

     This product was previously labelled as Protein Kinase D2

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245527 was affinity purified using an epitope specific to PKD2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/1000 - 1/5000. Predicted molecular weight: 97 kDa.

Target

  • Function

    Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylation of Ser-876 correlates with the activation status of the kinase.
    Ser-706 is probably phosphorylated by PKC.
  • Cellular localization

    Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp586E0820 antibody
    • HSPC187 antibody
    • KPCD2_HUMAN antibody
    • nPKC D2 antibody
    • nPKC-D2 antibody
    • PKD2 antibody
    • PRKD 2 antibody
    • Prkd2 antibody
    • Protein kinase D2 antibody
    • Serine/threonine protein kinase D2 antibody
    • Serine/threonine-protein kinase D2 antibody
    see all

Images

  • All lanes : Anti-PKD2 antibody (ab245527) at 0.4 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 97 kDa


    Exposure time: 30 seconds


    Lysates prepared in NETN buffer.

  • PKD2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) with ab245527 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab245527 at 1 µg/ml.

    Lane 1: ab245527 (Batch 1) IP in HeLa whole cell lysate.

    Lane 2: ab245527 (Batch 2) IP in HeLa whole cell lysate.

    Lane 3: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

References

ab245527 has not yet been referenced specifically in any publications.

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