Overview

  • Product name
    Anti-PKD2 antibody [EP1495Y]
    See all PKD2 primary antibodies
  • Description
    Rabbit monoclonal [EP1495Y] to PKD2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human PKD2 aa 200-300. The exact sequence is proprietary.

  • Positive control
    • HeLa whole cell lysate (ab150035) or human breast carcinoma tissue. IF/ICC: HeLa cell line
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

     This product was previously labelled as Protein Kinase D2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    EP1495Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
      Contains 1 PH domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylation of Ser-876 correlates with the activation status of the kinase.
      Ser-706 is probably phosphorylated by PKC.
    • Cellular localization
      Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp586E0820 antibody
      • HSPC187 antibody
      • KPCD2_HUMAN antibody
      • nPKC D2 antibody
      • nPKC-D2 antibody
      • PKD2 antibody
      • PRKD 2 antibody
      • Prkd2 antibody
      • Protein kinase D2 antibody
      • Serine/threonine protein kinase D2 antibody
      • Serine/threonine-protein kinase D2 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: PKD2 knockout HAP1 cell lysate (40 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HEK293 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab51250 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab51250 was shown to recognize PKD2 when PKD2 knockout samples were used, along with additional cross-reactive bands. Wild-type and PKD2 knockout samples were subjected to SDS-PAGE. Ab51250 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

    • Anti-PKD2 antibody [EP1495Y] (ab51250) at 1/500 dilution + HeLa cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 97 kDa
      Observed band size: 105 kDa
      why is the actual band size different from the predicted?

    • Ab51250 (1:100) staining human PKD2 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

    • ICC/IF image of ab51250 stained HeLa cells. The cells were 4% Formaldehyde fixed (10 mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51250, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References

    This product has been referenced in:
    • Baker J  et al. Protein kinase D3 modulates MMP1 and MMP13 expression in human chondrocytes. PLoS One 13:e0195864 (2018). Read more (PubMed: 29652915) »
    • Plastira I  et al. Lysophosphatidic acid via LPA-receptor 5/protein kinase D-dependent pathways induces a motile and pro-inflammatory microglial phenotype. J Neuroinflammation 14:253 (2017). Read more (PubMed: 29258556) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-7 of 7 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (BV2 cells)
    Permeabilization
    Yes - TritonX
    Specification
    BV2 cells
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde

    Dr. Joanna Plastira

    Verified customer

    Submitted Oct 12 2018

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Primary murine microglia)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (7%)
    Loading amount
    100 µg
    Treatment
    Lysophosphatidic acid (LPA) 1µM
    Specification
    Primary murine microglia
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Dr. Joanna Plastira

    Verified customer

    Submitted Oct 12 2018

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Ileum)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Ileum
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Christopher Trummer

    Verified customer

    Submitted Oct 04 2018

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Ileum)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Ileum
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Christopher Trummer

    Verified customer

    Submitted Oct 04 2018

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Guinea pig Tissue lysate - whole (Ileum)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Ileum
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Christopher Trummer

    Verified customer

    Submitted Oct 04 2018

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (Ileum)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Ileum
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Christopher Trummer

    Verified customer

    Submitted Oct 04 2018

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Ileum)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Ileum
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Christopher Trummer

    Verified customer

    Submitted Oct 04 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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