Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1496Y] to PKD2 (phospho S876)
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-PKD2 (phospho S876) antibody [EP1496Y]
See all PKD2 primary antibodies
DescriptionRabbit monoclonal [EP1496Y] to PKD2 (phospho S876)
This antibody detects PKD2 when phosphorylated at Ser876.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human PKD2 aa 850-950 (phospho S876). The exact sequence is proprietary.
- HeLa cell lysate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Protein Kinase D2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab51251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).
Is unsuitable for Flow Cyt, IHC or ICC/IF.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionConverts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
modificationsPhosphorylation of Ser-876 correlates with the activation status of the kinase.
Ser-706 is probably phosphorylated by PKC.
Cellular localizationCytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
- Information by UniProt
- DKFZp586E0820 antibody
- HSPC187 antibody
- KPCD2_HUMAN antibody
All lanes : Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with TPA
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab51251 has been referenced in 2 publications.
- Liu Q et al. PRKD2 Promotes Progression and Chemoresistance of AML via Regulating Notch1 Pathway. Onco Targets Ther 12:10931-10941 (2019). PubMed: 31849496
- Bernhart E et al. Protein kinase D2 regulates migration and invasion of U87MG glioblastoma cells in vitro. Exp Cell Res 319:2037-48 (2013). PubMed: 23562655