Overview

  • Product name

    Anti-PKD2 (phospho S876) antibody [EP1496Y]
    See all PKD2 primary antibodies
  • Description

    Rabbit monoclonal [EP1496Y] to PKD2 (phospho S876)
  • Host species

    Rabbit
  • Specificity

    This antibody detects PKD2 when phosphorylated at Ser876.

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PKD2 aa 850-950 (phospho S876). The exact sequence is proprietary.

  • Positive control

    • HeLa cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Protein Kinase D2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EP1496Y
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: 1/40.
    WB: 1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).

    Is unsuitable for Flow Cyt, IHC or ICC/IF.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
      Contains 1 PH domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylation of Ser-876 correlates with the activation status of the kinase.
      Ser-706 is probably phosphorylated by PKC.
    • Cellular localization

      Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
    • Information by UniProt
    • Database links

    • Alternative names

      • DKFZp586E0820 antibody
      • HSPC187 antibody
      • KPCD2_HUMAN antibody
      • nPKC D2 antibody
      • nPKC-D2 antibody
      • PKD2 antibody
      • PRKD 2 antibody
      • Prkd2 antibody
      • Protein kinase D2 antibody
      • Serine/threonine protein kinase D2 antibody
      • Serine/threonine-protein kinase D2 antibody
      see all

    Images

    • All lanes : Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with TPA

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 97 kDa
      Observed band size: 105 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Bernhart E  et al. Protein kinase D2 regulates migration and invasion of U87MG glioblastoma cells in vitro. Exp Cell Res 319:2037-48 (2013). Read more (PubMed: 23562655) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (BV2 microglia)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    100 µg
    Treatment
    1µM LPA for 40min in the absence or presence of a PKD family inhibitor (CRT1µM)
    Specification
    BV2 microglia
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Dr. Lisha Joshi

    Verified customer

    Submitted Jun 12 2019

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Primary murine microglia)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (7%)
    Loading amount
    100 µg
    Treatment
    Lysophosphatidic acid (LPA) 1µM
    Specification
    Primary murine microglia
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Dr. Joanna Plastira

    Verified customer

    Submitted Oct 12 2018

    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Left ventricle)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Left ventricle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Christopher Trummer

    Verified customer

    Submitted Oct 04 2018

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Left Ventricle)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Left Ventricle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Christopher Trummer

    Verified customer

    Submitted Oct 04 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up