Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1496Y] to PKD2 (phospho S876) - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-PKD2 (phospho S876) antibody [EP1496Y] - BSA and Azide free
See all PKD2 primary antibodies
DescriptionRabbit monoclonal [EP1496Y] to PKD2 (phospho S876) - BSA and Azide free
This antibody detects PKD2 when phosphorylated at Ser876.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human PKD2 aa 850-950 (phospho S876). The exact sequence is proprietary.
- HeLa cell lysate.
ab246493 is the carrier-free version of ab51251 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab246493 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Protein Kinase D2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab246493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).|
|IP||Use at an assay dependent concentration.|
FunctionConverts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
modificationsPhosphorylation of Ser-876 correlates with the activation status of the kinase.
Ser-706 is probably phosphorylated by PKC.
Cellular localizationCytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
- Information by UniProt
- DKFZp586E0820 antibody
- HSPC187 antibody
- KPCD2_HUMAN antibody
All lanes : Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with TPA
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
This data was developed using ab51251, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246493 has not yet been referenced specifically in any publications.