Overview

  • Product name

    Anti-PKE antibody
  • Description

    Rabbit polyclonal to PKE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Human PKE aa 102-419.

  • Positive control

    • A431 cells; A431, Raji, MOLT4 and Mouse brain lysates; Human A549 xenograft tissue.
  • General notes

    Protein previously labeled as STK32C.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

Images

  • Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling PKE with ab153917 at 1/500 dilution.

  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PKE with ab153917 at 1/100 dilution. Lower panel is merged with DNA probe.

  • Anti-PKE antibody (ab153917) at 1/500 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 55 kDa



    7.5% SDS PAGE
  • Anti-PKE antibody (ab153917) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 55 kDa



    10% SDS PAGE

References

ab153917 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153917.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up