Key features and details
- Rat monoclonal [8G12A1] to PKHD1
- Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-PKHD1 antibody [8G12A1]
See all PKHD1 primary antibodies
DescriptionRat monoclonal [8G12A1] to PKHD1
Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Mouse PKHD1 aa 3878-4060.
TAW NAQKKHCELE RHLSTEQFLP NLGSTVPGEN YFDRTYQMLY LFLKGTTPVE VHTATVIFVS FHLPVMTADE FFSSHNLVRN LALFLKIPSD KIRVSRIIGA SLRKKRSTGH IMEFEIGAAP TQFLSNSTTG QMQLSELQEI TDSLGQAVVL GKISTILGFN ISSMSITSPI PQPTDSGWIK
Database link: Q80ZA4
- Mouse recombinant protein, A431 cells, HeLa cells, esophageal cancer tissue.
This product was changed from ascites to supernatant. Lot no’s high than GR291788-5 are from Tissue Culture Supernatant
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab204951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 465 kDa.|
|ICC/IF||1/200 - 1/1000.|
|Flow Cyt||1/200 - 1/400.|
|IHC-P||1/200 - 1/1000.|
FunctionMay be a receptor protein that acts in collecting-duct and biliary differentiation.
Tissue specificityPredominantly expressed in fetal and adult kidney. Also present in the adult pancreas, but at much lower levels. Detectable in fetal and adult liver. Rather indistinct signal in fetal brain.
Involvement in diseaseDefects in PKHD1 are the cause of polycystic kidney disease autosomal recessive (ARPKD) [MIM:263200]. ARPKD is a severe form of polycystic kidney disease affecting the kidneys and the hepatic biliary tract. The clinical spectrum is widely variable, with most cases presenting during infancy. The fetal phenotypic features classically include enlarged and echogenic kidneys, as well as oligohydramnios secondary to a poor urine output. Up to 50% of the affected neonates die shortly after birth, as a result of severe pulmonary hypoplasia and secondary respiratory insufficiency. In the subset that survives the perinatal period, morbidity and mortality are mainly related to severe systemic hypertension, renal insufficiency, and portal hypertension due to portal-tract fibrosis.
Sequence similaritiesContains 2 G8 domains.
Contains 12 IPT/TIG domains.
Contains 9 PbH1 repeats.
- Information by UniProt
- ARPKD antibody
- FCYT antibody
- Fibrocystin antibody
Immunofluorescent analysis of A431 cells labeling PKHD1 with ab204951 at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow cytometric analysis of HeLa cells labeling PKHD1 with ab204951 at 1/200 (green) or negative control (red)
Immunohistochemical analysis of paraffin-embedded esophageal cancer tissue labeling PKHD1 with ab204951 at 1/200 dilution followed by DAB staining
ab204951 has not yet been referenced specifically in any publications.