Anti-PKIB antibody (ab196689)
Key features and details
- Rabbit polyclonal to PKIB
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PKIB antibody
See all PKIB primary antibodies -
Description
Rabbit polyclonal to PKIB -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human PKIB (internal sequence).
Database link: Q9C010 -
Positive control
- Human brain tissue; MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab196689 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/500.
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|
IHC-P |
1/50 - 1/100.
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Notes |
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ICC/IF
1/100 - 1/500. |
IHC-P
1/50 - 1/100. |
Target
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Function
Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains. -
Sequence similarities
Belongs to the PKI family. - Information by UniProt
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Database links
- Entrez Gene: 5570 Human
- Entrez Gene: 18768 Mouse
- Omim: 606914 Human
- SwissProt: Q9C010 Human
- SwissProt: Q04758 Mouse
- Unigene: 726217 Human
- Unigene: 262135 Mouse
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Alternative names
- cAMP dependent protein kinase inhibitor 2 antibody
- cAMP-dependent protein kinase inhibitor beta antibody
- FLJ23817 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PKIB with ab196689 at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.
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Immunofluorescent analysis of MCF7 cells labeling PKIB with ab196689 at 1/100 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab196689 has been referenced in 1 publication.
- Qin M et al. Expression of S100A8 is induced by interleukin-1a in TR146 epithelial cells through a mechanism involving CCAAT/enhancer binding protein ß. Mol Med Rep 19:2413-2420 (2019). PubMed: 30664211