- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-PKLR antibody
See all PKLR primary antibodies -
DescriptionRabbit polyclonal to PKLR
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment containing a sequence corresponding to a region within amino acids 178-510 of Human PKLR.(Uniprot:P30613)
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Positive control
- A549 cell lysate, HeLa cell lysate, HepG2 cell lysate, HTC116 cell lysate and Human breast cancer tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab125697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 62 kDa. | |
| IHC-P | 1/100 - 1/500. Perfrom antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer(pH8.0). | |
| ICC/IF | 1/100 - 1/500. |
Target
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FunctionPlays a key role in glycolysis.
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PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
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Involvement in diseaseDefects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia. -
Sequence similaritiesBelongs to the pyruvate kinase family.
- Information by UniProt
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Database links
- Entrez Gene: 5313 Human
- Omim: 609712 Human
- SwissProt: P30613 Human
- Unigene: 95990 Human
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Alternative names
- EC 2.7.1.40 antibody
- KPYR_HUMAN antibody
- L-PK antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody (ab125697)ab125697, at 1/250 dilution, staining PKLR in Formalin-fixed, Paraffin-embedded Human breast cancer tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-PKLR antibody (ab125697)ab125697, at 1/500 dilution, staining PKLR in Methanol-fixed HeLa cells by Immunocytochemistry/Immunofluorescence. -
Western blot - Anti-PKLR antibody (ab125697)Anti-PKLR antibody (ab125697) at 1/1000 dilution + A549 cell lysate at 30 µg
Predicted band size: 62 kDa
Protocols
References
ab125697 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"