Key features and details
- Rabbit polyclonal to PKLR
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PKLR antibody
See all PKLR primary antibodies
DescriptionRabbit polyclonal to PKLR
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment containing a sequence corresponding to a region within amino acids 178-510 of Human PKLR.(Uniprot:P30613)
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab125697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 62 kDa.|
|IHC-P||1/100 - 1/500. Perfrom antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer(pH8.0).|
|ICC/IF||1/100 - 1/500.|
FunctionPlays a key role in glycolysis.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Involvement in diseaseDefects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia.
Sequence similaritiesBelongs to the pyruvate kinase family.
- Information by UniProt
- EC 22.214.171.124 antibody
- KPYR_HUMAN antibody
- L-PK antibody
Anti-PKLR antibody (ab125697) at 1/1000 dilution + A549 cell lysate at 30 µg
Predicted band size: 62 kDa
ab125697, at 1/250 dilution, staining PKLR in Formalin-fixed, Paraffin-embedded Human breast cancer tissue by Immunohistochemistry.
ab125697, at 1/500 dilution, staining PKLR in Methanol-fixed HeLa cells by Immunocytochemistry/Immunofluorescence.
ab125697 has not yet been referenced specifically in any publications.