Product nameAnti-PKLR antibody
See all PKLR primary antibodies
DescriptionRabbit polyclonal to PKLR
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Mouse, Human
- Breast carcinoma tissue and NIH/3T3 cell lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab38240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionPlays a key role in glycolysis.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Involvement in diseaseDefects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia.
Sequence similaritiesBelongs to the pyruvate kinase family.
- Information by UniProt
- EC 220.127.116.11 antibody
- KPYR_HUMAN antibody
- L-PK antibody
Anti-PKLR antibody (ab38240) at 1/100 dilution + NIH/3T3 cell lysate
Predicted band size: 62 kDa
Observed band size: 62 kDa
Additional bands at: 30 kDa, 50 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Formalin-fixed and paraffin-embedded human breast cancer tissue staineded with ab38240 at a 1/50 dilution, using a peroxidase-conjugated secondary antibody, followed by AEC staining.
This data demonstrates the suitability of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
This product has been referenced in:
- Zhang CS et al. Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK. Nature 548:112-116 (2017). Read more (PubMed: 28723898) »
- Li YG et al. 1-deoxynojirimycin inhibits glucose absorption and accelerates glucose metabolism in streptozotocin-induced diabetic mice. Sci Rep 3:1377 (2013). WB ; Mouse . Read more (PubMed: 23536174) »