Key features and details
- Mouse polyclonal to PKLR
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PKLR antibody
See all PKLR primary antibodies
DescriptionMouse polyclonal to PKLR
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Full length Human PKLR protein, amino acids 1-574 (NP_000289.1)
- Human liver tissue lysate, PKLR transfected 293T cell lysate, Human kidney tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab89071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays a key role in glycolysis.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Involvement in diseaseDefects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia.
Sequence similaritiesBelongs to the pyruvate kinase family.
- Information by UniProt
- EC 220.127.116.11 antibody
- KPYR_HUMAN antibody
- L-PK antibody
Anti-PKLR antibody (ab89071) at 1 µg/ml + Human liver tissue lysate at 50 µg
Predicted band size: 62 kDa
Observed band size: 62 kDa
All lanes : Anti-PKLR antibody (ab89071) at 1 µg/ml
Lane 1 : PKLR transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 62 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
ab89071 at 3µg/ml staining PKLR in Human kidney by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
ab89071 has been referenced in 1 publication.
- Kung C et al. AG-348 enhances pyruvate kinase activity in red blood cells from patients with pyruvate kinase deficiency. Blood 130:1347-1356 (2017). PubMed: 28760888