Product nameAnti-PKLR antibody [EPR11093]
See all PKLR primary antibodies
DescriptionRabbit monoclonal [EPR11093] to PKLR
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PKLR aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: P30613
- HT-29, HeLa, HepG2 and 293T cell lysates; Human kidney and liver tissues.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 62 kDa.|
|IHC-P||1/250 - 1/500.|
FunctionPlays a key role in glycolysis.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Involvement in diseaseDefects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia.
Sequence similaritiesBelongs to the pyruvate kinase family.
- Information by UniProt
- EC 22.214.171.124 antibody
- KPYR_HUMAN antibody
- L-PK antibody
Immunohistochemistry of paraffin-embedded Human liver tissue labeling PKLR using ab171744 at 1/250 dilution.
Immunohistochemistry of paraffin-embedded Human kidney tissue labeling PKLR using ab171744 at 1/250 dilution.
All lanes : Anti-PKLR antibody [EPR11093] (ab171744) at 1/1000 dilution
Lane 1 : HT-29 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 62 kDa
ab171744 has not yet been referenced specifically in any publications.