Recombinant
RabMAb

Anti-PKLR antibody [EPR11093] (ab171744)

Overview

  • Product name
    Anti-PKLR antibody [EPR11093]
    See all PKLR primary antibodies
  • Description
    Rabbit monoclonal [EPR11093] to PKLR
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PKLR aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: P30613

  • Positive control
    • HT-29, HeLa, HepG2 and 293T cell lysates; Human kidney and liver tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 62 kDa.
IHC-P 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Plays a key role in glycolysis.
    • Pathway
      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
    • Involvement in disease
      Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
      Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia.
    • Sequence similarities
      Belongs to the pyruvate kinase family.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 2.7.1.40 antibody
      • KPYR_HUMAN antibody
      • L-PK antibody
      • Pk-1 antibody
      • PK1 antibody
      • PKL antibody
      • Pklg antibody
      • Pklr antibody
      • PKR antibody
      • PKRL antibody
      • Pyruvate kinase 1 antibody
      • Pyruvate kinase isozymes R/L antibody
      • Pyruvate kinase liver and blood cell antibody
      • Pyruvate kinase liver and RBC antibody
      • Pyruvate kinase liver and red blood cell antibody
      • Pyruvate kinase liver type antibody
      • Pyruvate kinase type L antibody
      • Pyruvate kinase, red cell type antibody
      • R type/L type pyruvate kinase antibody
      • R-PK antibody
      • R-type/L-type pyruvate kinase antibody
      • Red cell/liver pyruvate kinase antibody
      • RPK antibody
      see all

    Images

    • Immunohistochemistry of paraffin-embedded Human liver tissue labeling PKLR using ab171744 at 1/250 dilution.

    • Immunohistochemistry of paraffin-embedded Human kidney tissue labeling PKLR using ab171744 at 1/250 dilution.

    • All lanes : Anti-PKLR antibody [EPR11093] (ab171744) at 1/1000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 62 kDa

    References

    ab171744 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab171744.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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