Overview

  • Product name

  • Description

    Rabbit polyclonal to PKM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human PKM aa 243-531.
    Database link: P14618

  • Positive control

    • Recombinant Human PKM2 protein (ab53384) can be used as a positive control in WB. A431, H1299, HepG2, MOLT4, Raji, 293T, HeLaS3 and Mouse brain whole cell lysates; HeLa cells; Human Cal27 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • Tissue specificity

    Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • Sequence similarities

    Belongs to the pyruvate kinase family.
  • Post-translational
    modifications

    ISGylated.
    Under hypoxia, hydroxylated by EGLN3.
    Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
    FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
  • Information by UniProt
  • Database links

  • Alternative names

    • CTHBP antibody
    • Cytosolic thyroid hormone-binding protein antibody
    • KPYM_HUMAN antibody
    • OIP-3 antibody
    • Opa-interacting protein 3 antibody
    • p58 antibody
    • pkm antibody
    • PKM1 antibody
    • PKM2 antibody
    • Pyruvate kinase 2/3 antibody
    • Pyruvate kinase muscle isozyme antibody
    • Pyruvate kinase PKM antibody
    • THBP1 antibody
    • Thyroid hormone-binding protein 1 antibody
    • Tumor M2-PK antibody
    see all

Images

  • All lanes : Anti-PKM antibody (ab137791) at 1/5000 dilution

    Lane 1 : Non-transfected (-) 293T whole cell extracts
    Lane 2 : 293T whole cell extracts transfected (+) with PKM shRNA

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 58 kDa

  • Immunofluorescence analysis of methanol-fixed HeLa cells labelling PKM with ab137791 at 1/500 dilution. The lower image is co-stained with Hoechst 33342.

  • All lanes : Anti-PKM antibody (ab137791) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HeLa S3 whole cell lysate


    Lysates/proteins at 30 µg per lane.

    Predicted band size: 58 kDa



    7.5% SDS PAGE
  • Anti-PKM antibody (ab137791) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 58 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labelling PKM with ab137791 at 1/500 dilution.

References

This product has been referenced in:

  • Xu Q  et al. Adipose tissue proteomic analysis in ketotic or healthy Holstein cows in early lactation. J Anim Sci N/A:N/A (2019). Read more (PubMed: 30985866) »
  • Li JH  et al. Over expression of hyaluronan promotes progression of HCC via CD44-mediated pyruvate kinase M2 nuclear translocation. Am J Cancer Res 6:509-21 (2016). Read more (PubMed: 27186420) »
See all 2 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Rat Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 01 2017

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Brain
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 19 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, liver, retina, RPE)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Brain, liver, retina, RPE
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 19 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK, ARPE19)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
HEK, ARPE19
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 19 2017

Application
Western blot
Sample
Xenopus laevis Cell lysate - other (loaded 0.5 laevis embryo 1trop embryo not 1000 µg)
Gel Running Conditions
Reduced Denaturing
Loading amount
1000 µg
Specification
loaded 0.5 laevis embryo 1trop embryo not 1000 µg
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Shoko Ishibashi

Verified customer

Submitted Apr 26 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Tissue lysate - whole (skeletal muscle)
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Rafael Montes Nieto

Verified customer

Submitted Oct 30 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up