Question (55907) | Anti-PKM antibody (ab38237)

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Question

Trying to get antibody to work in a non-reducing/non-denaturing western blot. Any protocol advice?

Answer

Thank you for contacting Abcam.

I have been talking to my colleagues and we cannot think of any good ways to get an antibody to work in non-reduced, non-denatured conditions, they either work in that application or not.

I am sorry that I could not be more help in this matter, but if there is anything else I can help you with, please let me know.

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