Western blot abreview for Anti-PKM2 antibody

Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% Acrylamide gel)
Sample
Human Cell lysate - whole cell (MDA-MB-231 breast cancer cell line)
Specification
MDA-MB-231 breast cancer cell line
Treatment
TGF beta 2.5ng/ml-1 3 hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: PBST 5% milk
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: DAKO (P0448)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Bands
Specific: ~58, 64, 68 kDa
Exposure
3 minute(s) and 0 second(s)

Wendy Greenwood

Verified customer

Submitted Mar 23 2015

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