Immunohistochemistry (Frozen sections) abreview for Anti-PKM antibody

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Ovary)
Permeabilization
No
Specification
Ovary
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-BSAc
Dilution
1/100

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Cross-Adsbd Secondary
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
Heat-mediated antigen retrieval (10 mM Sodium Citrate buffer, pH 6.0) - 10 minutes sub-boiling. Glycine incubation (50 mM) - 20 minutes.
Abcam response
The immunogen shows more than 97% similarity with porcine sequence.

Ms. Natasja Costermans

Verified customer

Submitted Oct 17 2018

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