Overview

  • Product name
  • Description
    Rabbit polyclonal to PKM
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PKM (C terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control
    • Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab76695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/1000.

Target

  • Function
    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • Tissue specificity
    Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • Sequence similarities
    Belongs to the pyruvate kinase family.
  • Post-translational
    modifications
    ISGylated.
    Under hypoxia, hydroxylated by EGLN3.
    Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
    FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • CTHBP antibody
    • Cytosolic thyroid hormone-binding protein antibody
    • KPYM_HUMAN antibody
    • OIP-3 antibody
    • Opa-interacting protein 3 antibody
    • p58 antibody
    • pkm antibody
    • PKM1 antibody
    • PKM2 antibody
    • Pyruvate kinase 2/3 antibody
    • Pyruvate kinase muscle isozyme antibody
    • Pyruvate kinase PKM antibody
    • THBP1 antibody
    • Thyroid hormone-binding protein 1 antibody
    • Tumor M2-PK antibody
    see all

Images

  • ab76695 at 1/50 dilution, staining PKM in human breast carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.

References

ab76695 has not yet been referenced specifically in any publications.

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