Overview

  • Product name
  • Description
    Goat polyclonal to PKM
  • Host species
    Goat
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rabbit, Human
    Predicted to work with: Mammals
  • Immunogen

    Full length native protein (purified) corresponding to Rabbit PKM. Purified from muscle tissue.

  • Positive control
    • Uterus tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab79070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • Tissue specificity
    Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • Sequence similarities
    Belongs to the pyruvate kinase family.
  • Post-translational
    modifications
    ISGylated.
    Under hypoxia, hydroxylated by EGLN3.
    Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
    FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • CTHBP antibody
    • Cytosolic thyroid hormone-binding protein antibody
    • KPYM_HUMAN antibody
    • OIP-3 antibody
    • Opa-interacting protein 3 antibody
    • p58 antibody
    • pkm antibody
    • PKM1 antibody
    • PKM2 antibody
    • Pyruvate kinase 2/3 antibody
    • Pyruvate kinase muscle isozyme antibody
    • Pyruvate kinase PKM antibody
    • THBP1 antibody
    • Thyroid hormone-binding protein 1 antibody
    • Tumor M2-PK antibody
    see all

Images

  • ab79070, at 5µg/ml, staining PKM in uterus tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

References

ab79070 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10000µg/mL · Temperature: 25°C
Sample
Human Cell (Cholangiocarcinoma cell)
Specification
Cholangiocarcinoma cell
Permeabilization
Yes - 0.25% TritonX-100
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 26 2014

Question
Answer



J’ai contacté le laboratoire est malheureusement l’épitope d’ab79070 n’a pas été déterminé.

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (ECR 293 cell line)
Specification
ECR 293 cell line
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 25°C

Dr. Ismail Hendaoui

Verified customer

Submitted Feb 04 2013

Question
Answer

Merci pour votre appel de hier.

La laboratoire à pu me confirmer, que pour l'IP1 x 107 cellules ont été lysées et resuspendues en 0.2ml. Ensuite, ils ont ajouté de 0.5à 5 ul de cet anticorps et incubé le mélange pour une heure.

Je peux donc vous conseiller d'utiliser plutôt 0.5ul/107 cellules si PKM2 est abondant dans vos cellules. Si vous pensez qu'il n'y a pas beaucoup de PKM2 dans vos cellules, je vous conseiller de plutôt utilser 5ul/107 cellules.

Voici aussi un lien pour notre protocole générique pour l'IP: https://www.abcam.com/index.html?pageconfig=resource&rid=11385

J'espère que cette information vous est utile. N'hésitez pas à me recontacter si vous avez des autres questions.

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