Recombinant Anti-PKM antibody [EPR10138(B)] (ab150377)
- Datasheet
- References (8)
- Protocols
Overview
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Product name
Anti-PKM antibody [EPR10138(B)]
See all PKM primary antibodies -
Description
Rabbit monoclonal [EPR10138(B)] to PKM -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig -
Immunogen
Synthetic peptide within Human PKM aa 50-150. The exact sequence is proprietary.
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Positive control
- Recombinant human PKM2 protein (ab89364) can be used as a positive control in WB. Human skeletal muscle, HeLa, Jurkat and A549 cell lysates; HeLa cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10138(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab150377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/500. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 58 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. -
Tissue specificity
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
Sequence similarities
Belongs to the pyruvate kinase family. -
Post-translational
modificationsISGylated.
Under hypoxia, hydroxylated by EGLN3.
Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity. - Information by UniProt
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Database links
- Entrez Gene: 5315 Human
- SwissProt: P14618 Human
- Unigene: 534770 Human
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Alternative names
- CTHBP antibody
- Cytosolic thyroid hormone-binding protein antibody
- KPYM_HUMAN antibody
see all
Images
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![Western blot - Anti-PKM antibody [EPR10138(B)] (ab150377)](https://www.abcam.com/ps/products/150/ab150377/Images/ab150377-156963-anti-pkm-antibody-epr10138b-western-blot.jpg)
Western blot - Anti-PKM antibody [EPR10138(B)] (ab150377)All lanes : Anti-PKM antibody [EPR10138(B)] (ab150377) at 1/1000 dilution
Lane 1 : Human skeletal muscle cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 58 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody [EPR10138(B)] (ab150377)](https://www.abcam.com/ps/products/150/ab150377/Images/ab150377-265174-anti-pkm-antibody-epr10138b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody [EPR10138(B)] (ab150377)Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling PKM with purified ab150377 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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![Flow Cytometry - Anti-PKM antibody [EPR10138(B)] (ab150377)](https://www.abcam.com/ps/products/150/ab150377/Images/ab150377-156962-anti-pkm-antibody-epr10138b-flow-cytometry.jpg)
Flow Cytometry - Anti-PKM antibody [EPR10138(B)] (ab150377)Flow cytometric analysis of permeabilized HeLa cells labeling PKM with ab150377 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
References
This product has been referenced in:
- Ouyang X et al. Metabolic regulation of T cell development by Sin1-mTORC2 is mediated by pyruvate kinase M2. J Mol Cell Biol 11:93-106 (2019). Read more (PubMed: 30428057) »
- Wang SQ et al. Heterozygous knockout insulin-like growth factor-1 receptor (IGF-1R) regulates mitochondrial functions and prevents colitis and colorectal cancer. Free Radic Biol Med 134:87-98 (2019). Read more (PubMed: 30611867) »
