Overview

  • Product name

    Anti-PKM antibody [EPR10138(B)]
    See all PKM primary antibodies
  • Description

    Rabbit monoclonal [EPR10138(B)] to PKM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide within Human PKM aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Recombinant human PKM2 protein (ab89364) can be used as a positive control in WB. Human skeletal muscle, HeLa, Jurkat and A549 cell lysates; HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
    • Tissue specificity

      Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
    • Pathway

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
    • Sequence similarities

      Belongs to the pyruvate kinase family.
    • Post-translational
      modifications

      ISGylated.
      Under hypoxia, hydroxylated by EGLN3.
      Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
      FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
    • Cellular localization

      Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
    • Information by UniProt
    • Database links

    • Alternative names

      • CTHBP antibody
      • Cytosolic thyroid hormone-binding protein antibody
      • KPYM_HUMAN antibody
      • OIP-3 antibody
      • Opa-interacting protein 3 antibody
      • p58 antibody
      • pkm antibody
      • PKM1 antibody
      • PKM2 antibody
      • Pyruvate kinase 2/3 antibody
      • Pyruvate kinase muscle isozyme antibody
      • Pyruvate kinase PKM antibody
      • THBP1 antibody
      • Thyroid hormone-binding protein 1 antibody
      • Tumor M2-PK antibody
      see all

    Images

    • All lanes : Anti-PKM antibody [EPR10138(B)] (ab150377) at 1/1000 dilution

      Lane 1 : Human skeletal muscle cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size: 58 kDa

    • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling PKM with purified ab150377 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

    • Flow cytometric analysis of permeabilized HeLa cells labeling PKM with ab150377 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Ouyang X  et al. Metabolic regulation of T cell development by Sin1-mTORC2 is mediated by pyruvate kinase M2. J Mol Cell Biol 11:93-106 (2019). Read more (PubMed: 30428057) »
    • Wang SQ  et al. Heterozygous knockout insulin-like growth factor-1 receptor (IGF-1R) regulates mitochondrial functions and prevents colitis and colorectal cancer. Free Radic Biol Med 134:87-98 (2019). Read more (PubMed: 30611867) »
    See all 8 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jun 30 2015

    Application
    Western blot
    Loading amount
    80 µg
    Gel Running Conditions
    Reduced Denaturing (12.5% Acrylamide, 2D gel ph 3-10)
    Sample
    Pig Tissue lysate - other (Muscle Sarcoplasm)
    Specification
    Muscle Sarcoplasm
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Dr. Shannon Cruzen

    Verified customer

    Submitted Sep 29 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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