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  1. Link

    pkm-antibody-epr10138b-ab150377.pdf

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Signal Transduction Metabolism Energy Metabolism
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RecombinantRabMAb

Recombinant Anti-PKM antibody [EPR10138(B)] (ab150377)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (16)

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Flow Cytometry (Intracellular) - Anti-PKM antibody [EPR10138(B)] (ab150377)
  • Western blot - Anti-PKM antibody [EPR10138(B)] (ab150377)
  • Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody [EPR10138(B)] (ab150377)
  • Anti-PKM antibody [EPR10138(B)] (ab150377)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10138(B)] to PKM
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free PE

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Overview

  • Product name

    Anti-PKM antibody [EPR10138(B)]
    See all PKM primary antibodies
  • Description

    Rabbit monoclonal [EPR10138(B)] to PKM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide within Human PKM aa 50-150. The exact sequence is proprietary.
    Database link: P14618

  • Positive control

    • WB: HeLa, Jurkat, A549, Mouse lung, human skeletal muscle, rat skeletal muscle, mouse skeletal muscle lysates
  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10138(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Other
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (ab206129)
    • PE Anti-PKM antibody [EPR10138(B)] (ab210448)
    • Alexa Fluor® 647 Anti-PKM antibody [EPR10138(B)] (ab214257)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
    • A549 whole cell lysate (ab7910)
    • Recombinant human PKM2 protein (Active) (ab89364)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF (1)
1/50.
WB (2)
1/10000. Predicted molecular weight: 58 kDa.
Notes
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/50.
WB
1/10000. Predicted molecular weight: 58 kDa.

Target

  • Function

    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • Tissue specificity

    Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • Sequence similarities

    Belongs to the pyruvate kinase family.
  • Post-translational
    modifications

    ISGylated.
    Under hypoxia, hydroxylated by EGLN3.
    Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
    FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
  • Target information above from: UniProt accession P14618 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5315 Human
    • SwissProt: P14618 Human
    • Unigene: 534770 Human
    • Alternative names

      • CTHBP antibody
      • Cytosolic thyroid hormone-binding protein antibody
      • KPYM_HUMAN antibody
      • OIP-3 antibody
      • Opa-interacting protein 3 antibody
      • p58 antibody
      • pkm antibody
      • PKM1 antibody
      • PKM2 antibody
      • Pyruvate kinase 2/3 antibody
      • Pyruvate kinase muscle isozyme antibody
      • Pyruvate kinase PKM antibody
      • THBP1 antibody
      • Thyroid hormone-binding protein 1 antibody
      • Tumor M2-PK antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Anti-PKM antibody [EPR10138(B)] (ab150377)
      Flow Cytometry (Intracellular) - Anti-PKM antibody [EPR10138(B)] (ab150377)

      Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling PKM with Purified ab150377 at 1:50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Western blot - Anti-PKM antibody [EPR10138(B)] (ab150377)
      Western blot - Anti-PKM antibody [EPR10138(B)] (ab150377)
      All lanes : Anti-PKM antibody [EPR10138(B)] (ab150377) at 1/10000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
      Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
      Lane 4 : Mouse lung lysate
      Lane 5 : Human skeletal muscle lysate
      Lane 6 : Rat skeletal muscle lysate
      Lane 7 : Mouse skeletal muscle lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 58 kDa
      Observed band size: 58 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody [EPR10138(B)] (ab150377)
      Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody [EPR10138(B)] (ab150377)
      Immunocytochemistry analysis of A549(Human lung carcinoma epithelial cell) cells labeling PKM with Purified ab150377 at 1/50 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A+H10:L10] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Anti-PKM antibody [EPR10138(B)] (ab150377)
      Anti-PKM antibody [EPR10138(B)] (ab150377)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (16)

    Publishing research using ab150377? Please let us know so that we can cite the reference in this datasheet.

    ab150377 has been referenced in 16 publications.

    • Sharma R  et al. Determinants of resistance to VEGF-TKI and immune checkpoint inhibitors in metastatic renal cell carcinoma. J Exp Clin Cancer Res 40:186 (2021). PubMed: 34099013
    • Cen S  et al. TRAF4 acts as a fate checkpoint to regulate the adipogenic differentiation of MSCs by activating PKM2. EBioMedicine 54:102722 (2020). PubMed: 32268273
    • Tian X  et al. Noscapine Induces Apoptosis in Human Colon Cancer Cells by Regulating Mitochondrial Damage and Warburg Effect via PTEN/PI3K/mTOR Signaling Pathway. Onco Targets Ther 13:5419-5428 (2020). PubMed: 32606759
    • Wang C  et al. Autophagy activated by silibinin contributes to glioma cell death via induction of oxidative stress-mediated BNIP3-dependent nuclear translocation of AIF. Cell Death Dis 11:630 (2020). PubMed: 32801360
    • Ouyang X  et al. Metabolic regulation of T cell development by Sin1-mTORC2 is mediated by pyruvate kinase M2. J Mol Cell Biol 11:93-106 (2019). PubMed: 30428057
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-PKM antibody [EPR10138(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U87 Glioblastoma cell line)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    30 µg
    Treatment
    Flavopiridol
    Specification
    U87 Glioblastoma cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    PROF. Michele D'angelo

    Verified customer

    Submitted Feb 13 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PKM2 antibody [EPR10138(B)]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    Submitted Jun 30 2015

    Western blot abreview for Anti-PKM2 antibody [EPR10138(B)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    80 µg
    Gel Running Conditions
    Reduced Denaturing (12.5% Acrylamide, 2D gel ph 3-10)
    Sample
    Pig Tissue lysate - other (Muscle Sarcoplasm)
    Specification
    Muscle Sarcoplasm
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Shannon Cruzen

    Verified customer

    Submitted Sep 29 2014

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