Recombinant Anti-PKM antibody [EPR10138(B)] (ab150377)
![Flow Cytometry (Intracellular) - Anti-PKM antibody [EPR10138(B)] (ab150377)](https://www.abcam.com/ps/products/150/ab150377/Images/ab150377-9-anti-pkm-antibody-epr10138-b-flowcytometry-hela-human.jpg "Flow Cytometry (Intracellular) - Anti-PKM antibody [EPR10138(B)] (ab150377)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10138(B)] to PKM
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PKM antibody [EPR10138(B)]
See all PKM primary antibodies -
Description
Rabbit monoclonal [EPR10138(B)] to PKM -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig
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Immunogen
Synthetic peptide within Human PKM aa 50-150. The exact sequence is proprietary.
Database link: P14618 -
Positive control
- WB: HeLa, Jurkat, A549, Mouse lung, human skeletal muscle, rat skeletal muscle, mouse skeletal muscle lysates
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General notes
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10138(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | (1) |
1/50.
|
| WB | (2) |
1/10000. Predicted molecular weight: 58 kDa.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
1/50. |
|
WB
1/10000. Predicted molecular weight: 58 kDa. |
Target
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Function
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. -
Tissue specificity
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
Sequence similarities
Belongs to the pyruvate kinase family. -
Post-translational
modificationsISGylated.
Under hypoxia, hydroxylated by EGLN3.
Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity. - Information by UniProt
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Database links
- Entrez Gene: 5315 Human
- SwissProt: P14618 Human
- Unigene: 534770 Human
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Alternative names
- CTHBP antibody
- Cytosolic thyroid hormone-binding protein antibody
- KPYM_HUMAN antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-PKM antibody [EPR10138(B)] (ab150377)
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling PKM with Purified ab150377 at 1:50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Western blot - Anti-PKM antibody [EPR10138(B)] (ab150377)](https://www.abcam.com/ps/products/150/ab150377/Images/ab150377-478323-anti-pkm-antibody-epr10138-b-westernblot-human-mouse-rat.jpg)
Western blot - Anti-PKM antibody [EPR10138(B)] (ab150377)All lanes : Anti-PKM antibody [EPR10138(B)] (ab150377) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 4 : Mouse lung lysate
Lane 5 : Human skeletal muscle lysate
Lane 6 : Rat skeletal muscle lysate
Lane 7 : Mouse skeletal muscle lysate
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody [EPR10138(B)] (ab150377)](https://www.abcam.com/ps/products/150/ab150377/Images/ab150377-7-anti-pkm-antibody-epr10138-b-immunocytochemistry-a549-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody [EPR10138(B)] (ab150377)Immunocytochemistry analysis of A549(Human lung carcinoma epithelial cell) cells labeling PKM with Purified ab150377 at 1/50 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A+H10:L10] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Anti-PKM antibody [EPR10138(B)] (ab150377)](https://www.abcam.com/ps/products/150/ab150377/Images/ab150377-4-benefits-of-recombinant-antibodies.png)
Anti-PKM antibody [EPR10138(B)] (ab150377)
Protocols
Datasheets and documents
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Datasheet download
References (16)
ab150377 has been referenced in 16 publications.
- Sharma R et al. Determinants of resistance to VEGF-TKI and immune checkpoint inhibitors in metastatic renal cell carcinoma. J Exp Clin Cancer Res 40:186 (2021). PubMed: 34099013
- Cen S et al. TRAF4 acts as a fate checkpoint to regulate the adipogenic differentiation of MSCs by activating PKM2. EBioMedicine 54:102722 (2020). PubMed: 32268273
- Tian X et al. Noscapine Induces Apoptosis in Human Colon Cancer Cells by Regulating Mitochondrial Damage and Warburg Effect via PTEN/PI3K/mTOR Signaling Pathway. Onco Targets Ther 13:5419-5428 (2020). PubMed: 32606759
- Wang C et al. Autophagy activated by silibinin contributes to glioma cell death via induction of oxidative stress-mediated BNIP3-dependent nuclear translocation of AIF. Cell Death Dis 11:630 (2020). PubMed: 32801360
- Ouyang X et al. Metabolic regulation of T cell development by Sin1-mTORC2 is mediated by pyruvate kinase M2. J Mol Cell Biol 11:93-106 (2019). PubMed: 30428057
