Recombinant
RabMAb

Recombinant Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (ab206129)

Overview

  • Product name

    Anti-PKM antibody [EPR10138(B)] - BSA and Azide free
    See all PKM primary antibodies
  • Description

    Rabbit monoclonal [EPR10138(B)] to PKM - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide within Human PKM aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human PKM2 protein (ab53384) can be used as a positive control in WB. Human skeletal muscle, HeLa, Jurkat and A549 cell lysates; HeLa cells
  • General notes

    Ab206129 is the carrier-free version of ab150377. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab206129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
    • Tissue specificity

      Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
    • Pathway

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
    • Sequence similarities

      Belongs to the pyruvate kinase family.
    • Post-translational
      modifications

      ISGylated.
      Under hypoxia, hydroxylated by EGLN3.
      Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
      FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
    • Cellular localization

      Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
    • Information by UniProt
    • Database links

    • Alternative names

      • CTHBP antibody
      • Cytosolic thyroid hormone-binding protein antibody
      • KPYM_HUMAN antibody
      • OIP-3 antibody
      • Opa-interacting protein 3 antibody
      • p58 antibody
      • pkm antibody
      • PKM1 antibody
      • PKM2 antibody
      • Pyruvate kinase 2/3 antibody
      • Pyruvate kinase muscle isozyme antibody
      • Pyruvate kinase PKM antibody
      • THBP1 antibody
      • Thyroid hormone-binding protein 1 antibody
      • Tumor M2-PK antibody
      see all

    Images

    • This ICC data was generated using the same anti-PKM antibody clone, EPR10138(B), in a different buffer formulation (cat# ab150377).

      Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling PKM with purified ab150377 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

    • This Flow cytometry data was generated using the same anti-PKM antibody clone, EPR10138(B), in a different buffer formulation (cat# ab150377).

      Flow cytometric analysis of permeabilized HeLa cells labeling PKM with ab150377 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Xu B  et al. Bi-directional communication with the cumulus cells is involved in the deficiency of XY oocytes in the components essential for proper second meiotic spindle assembly. Dev Biol 385:242-52 (2014). Read more (PubMed: 24247007) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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