Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10138(B)] to PKM - BSA and Azide free
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human, Pig
Product nameAnti-PKM antibody [EPR10138(B)] - BSA and Azide free
See all PKM primary antibodies
DescriptionRabbit monoclonal [EPR10138(B)] to PKM - BSA and Azide free
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human, Pig
Synthetic peptide within Human PKM aa 50-150. The exact sequence is proprietary.
- Recombinant Human PKM2 protein (ab53384) can be used as a positive control in WB. Human skeletal muscle, HeLa, Jurkat and A549 cell lysates; HeLa cells
Ab206129 is the carrier-free version of ab150377. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab206129 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab206129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
FunctionGlycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Tissue specificitySpecifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Sequence similaritiesBelongs to the pyruvate kinase family.
Under hypoxia, hydroxylated by EGLN3.
Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
- Information by UniProt
- CTHBP antibody
- Cytosolic thyroid hormone-binding protein antibody
- KPYM_HUMAN antibody
This ICC data was generated using the same anti-PKM antibody clone, EPR10138(B), in a different buffer formulation (cat# ab150377).
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling PKM with purified ab150377 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab206129 has been referenced in 1 publication.
- Xu B et al. Bi-directional communication with the cumulus cells is involved in the deficiency of XY oocytes in the components essential for proper second meiotic spindle assembly. Dev Biol 385:242-52 (2014). PubMed: 24247007