Anti-PKM antibody - N-terminal (ab196560)
Key features and details
- Rabbit polyclonal to PKM - N-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PKM antibody - N-terminal
See all PKM primary antibodies -
Description
Rabbit polyclonal to PKM - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human PKM2 (N terminal).
Database link: P14618 -
Positive control
- Jurkat, HeLa and A549 extracts. Human, mouse and rat lung cancer tissue. HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/20 - 1/100.
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IHC-P |
1/50 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 58 kDa.
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Notes |
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ICC/IF
1/20 - 1/100. |
IHC-P
1/50 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 58 kDa. |
Target
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Function
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. -
Tissue specificity
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
Sequence similarities
Belongs to the pyruvate kinase family. -
Post-translational
modificationsISGylated.
Under hypoxia, hydroxylated by EGLN3.
Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity. - Information by UniProt
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Database links
- Entrez Gene: 5315 Human
- SwissProt: P14618 Human
- Unigene: 534770 Human
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Alternative names
- CTHBP antibody
- Cytosolic thyroid hormone-binding protein antibody
- KPYM_HUMAN antibody
see all
Images
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All lanes : Anti-PKM antibody - N-terminal (ab196560) at 1/500 dilution
Lane 1 : Jurkat extracts
Lane 2 : HeLa extracts
Lane 3 : A549 extracts
Predicted band size: 58 kDa -
Immunohistochemical analysis of paraffin-embedded human lung tissue labeling PKM2 with ab196560 at 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling PKM2 with ab196560 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling PKM2 with ab196560 at 1/100 dilution.
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Immunofluorescence analysis of HeLa cells labeling PKM2 using ab196560, at 1/20 dilution. Lower panel shows DAPI for nuclear staining (Blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196560 has not yet been referenced specifically in any publications.