Overview

  • Product name

    Anti-PKM antibody - N-terminal
    See all PKM primary antibodies
  • Description

    Rabbit polyclonal to PKM - N-terminal
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous level of total PKM protein.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human PKM2 (N terminal).
    Database link: P14618

  • Positive control

    • Jurkat, HeLa and A549 extracts. Human, mouse and rat lung cancer tissue. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab196560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/20 - 1/100.
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 58 kDa.

Target

  • Function

    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • Tissue specificity

    Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • Sequence similarities

    Belongs to the pyruvate kinase family.
  • Post-translational
    modifications

    ISGylated.
    Under hypoxia, hydroxylated by EGLN3.
    Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
    FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
  • Information by UniProt
  • Database links

  • Alternative names

    • CTHBP antibody
    • Cytosolic thyroid hormone-binding protein antibody
    • KPYM_HUMAN antibody
    • OIP-3 antibody
    • Opa-interacting protein 3 antibody
    • p58 antibody
    • pkm antibody
    • PKM1 antibody
    • PKM2 antibody
    • Pyruvate kinase 2/3 antibody
    • Pyruvate kinase muscle isozyme antibody
    • Pyruvate kinase PKM antibody
    • THBP1 antibody
    • Thyroid hormone-binding protein 1 antibody
    • Tumor M2-PK antibody
    see all

Images

  • All lanes : Anti-PKM antibody - N-terminal (ab196560) at 1/500 dilution

    Lane 1 : Jurkat extracts
    Lane 2 : HeLa extracts
    Lane 3 : A549 extracts

    Predicted band size: 58 kDa

  • Immunohistochemical analysis of paraffin-embedded human lung tissue labeling PKM2 with ab196560 at 1/50 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling PKM2 with ab196560 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling PKM2 with ab196560 at 1/100 dilution.

  • Immunofluorescence analysis of HeLa cells labeling PKM2 using ab196560, at 1/20 dilution. Lower panel shows DAPI for nuclear staining (Blue).

References

ab196560 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196560.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up