Key features and details
- Rabbit polyclonal to PKM2
- Suitable for: IHC-P, WB
- Reacts with: Zebrafish
- Isotype: IgG
Product nameAnti-PKM2 antibody
See all PKM2 primary antibodies
DescriptionRabbit polyclonal to PKM2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Zebrafish
Recombinant fragment within Zebrafish PKM2 aa 264-532. The exact sequence is proprietary.
Database link: 335817
- Whole Zebrafish and Zebrafish heart whole cell lysates; Zebrafish tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab210114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.
Suggested antigen retrieval using 1mM EDTA-NaOH (pH8.0) 95°C, 1 Hour
|WB||1/500 - 1/3000. Predicted molecular weight: 58 kDa.|
FunctionGlycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Tissue specificitySpecifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Sequence similaritiesBelongs to the pyruvate kinase family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
- Information by UniProt
- Entrez Gene: 335817 Zebrafish
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Immunohistochemical analysis of paraffin-embedded Zebrafish tissue labeling PKM2 with ab210114 at 1/300 dilution.
All lanes : Anti-PKM2 antibody (ab210114) at 1/1000 dilution
Lane 1 : Zebrafish whole cell lysate
Lane 2 : Zebrafish heart whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
7.5% SDS PAGE
ab210114 has not yet been referenced specifically in any publications.