Recombinant Anti-PKM antibody [EPR10139] (ab154816)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10139] to PKM
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PKM antibody [EPR10139]
See all PKM primary antibodies -
Description
Rabbit monoclonal [EPR10139] to PKM -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human PKM (internal sequence).
Database link: P14618 -
Positive control
- Human fetal muscle tissue lysate; HeLa; A549 and Jurkat cell lysates; Human kidney and cervical carcinoma tissue. IHC-P: Mouse and rat cerebrum. WB: Mouse and rat brain, testis and spleen tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10139 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. -
Tissue specificity
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
Sequence similarities
Belongs to the pyruvate kinase family. -
Post-translational
modificationsISGylated.
Under hypoxia, hydroxylated by EGLN3.
Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity. - Information by UniProt
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Database links
- Entrez Gene: 5315 Human
- SwissProt: P14618 Human
- Unigene: 534770 Human
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Alternative names
- CTHBP antibody
- Cytosolic thyroid hormone-binding protein antibody
- KPYM_HUMAN antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling PKM with ab154816 at 1/10000 (0.103 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Positive staining on rat cerebrum. The section was incubated with ab154816 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PKM with ab154816 at 1/10000 (0.103 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Positive staining on mouse cerebrum. The section was incubated with ab154816 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
All lanes : Anti-PKM antibody [EPR10139] (ab154816) at 1/1000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat testis tissue lysate
Lane 3 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
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All lanes : Anti-PKM antibody [EPR10139] (ab154816) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse testis tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
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All lanes : Anti-PKM antibody [EPR10139] (ab154816) at 1/1000 dilution
Lane 1 : Human fetal muscle tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 58 kDa -
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma, labeling PKM using ab154816 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human kidney, labeling PKM using ab154816 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154816 has been referenced in 2 publications.
- Wang JZ et al. The role of the HIF-1α/ALYREF/PKM2 axis in glycolysis and tumorigenesis of bladder cancer. Cancer Commun (Lond) 41:560-575 (2021). PubMed: 33991457
- Kimura A et al. FIR haplodeficiency promotes splicing to pyruvate kinase M2 in mice thymic lymphoma tissues revealed by six-plex tandem mass tag quantitative proteomic analysis. Oncotarget 8:67955-67965 (2017). PubMed: 28978087