Anti-PKMYT1 antibody (ab200387)
Key features and details
- Rabbit polyclonal to PKMYT1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PKMYT1 antibody
See all PKMYT1 primary antibodies -
Description
Rabbit polyclonal to PKMYT1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human PKMYT1 (internal sequence). The exact sequence is proprietary. NP_004194.
Database link: Q99640 -
Positive control
- Mouse heart tissue lysate; Human brain tissue.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/200 - 1/1000. Predicted molecular weight: 55 kDa.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/200 - 1/1000. Predicted molecular weight: 55 kDa. |
Target
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Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Contains 1 protein kinase domain. -
Domain
The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven. -
Post-translational
modificationsAutophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 9088 Human
- Entrez Gene: 268930 Mouse
- Omim: 602474 Human
- SwissProt: Q99640 Human
- SwissProt: Q9ESG9 Mouse
- Unigene: 77783 Human
- Unigene: 182193 Mouse
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Alternative names
- cdc2 inhibitory kinase antibody
- DKFZp547K1610 antibody
- FLJ20093 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PKMYT1 with ab200387 at 1/50 dilution.
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Anti-PKMYT1 antibody (ab200387) at 1/200 dilution + Mouse heart tissue lysate at 40 µg
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 2 minutesGel: 8%SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab200387 has not yet been referenced specifically in any publications.