Overview

  • Product name

  • Description

    Rabbit polyclonal to PKMYT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human PKMYT1 (internal sequence). The exact sequence is proprietary. NP_004194.
    Database link: Q99640

  • Positive control

    • Mouse heart tissue lysate; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab200387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/200 - 1/1000. Predicted molecular weight: 55 kDa.

Target

  • Function

    Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.
  • Post-translational
    modifications

    Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • cdc2 inhibitory kinase antibody
    • DKFZp547K1610 antibody
    • FLJ20093 antibody
    • Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase antibody
    • Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase antibody
    • MYT1 antibody
    • Myt1 kinase antibody
    • PKMYT 1 antibody
    • Pkmyt1 antibody
    • PMYT1_HUMAN antibody
    • PPP1R126 antibody
    • Protein kinase membrane associated tyrosine/threonine 1 antibody
    • Protein kinase Myt1 antibody
    • Protein phosphatase 1 regulatory subunit 126 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PKMYT1 with ab200387 at 1/50 dilution.

  • Anti-PKMYT1 antibody (ab200387) at 1/200 dilution + Mouse heart tissue lysate at 40 µg

    Developed using the ECL technique.

    Predicted band size: 55 kDa


    Exposure time: 2 minutes


    Gel: 8%SDS-PAGE

References

ab200387 has not yet been referenced specifically in any publications.

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