Product nameAnti-PKN antibody [EPR3237]
See all PKN primary antibodies
DescriptionRabbit monoclonal [EPR3237] to PKN
Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PKN. The exact sequence is proprietary.
- WB: Jurkat and Human spleen lysates IHC-P: Human brain tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended.
|ICC||1/100 - 1/250.|
RelevancePKN belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis.
- DBK antibody
- DPKN antibody
- PAK 1 antibody
All lanes : Anti-PKN antibody [EPR3237] (ab108976) at 1/1000 dilution
Lane 1 : Jurkat lysates
Lane 2 : Human spleen lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 104 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of PKN in paraffin embedded Human brain tissue, using ab108976 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab108976 has not yet been referenced specifically in any publications.