Product nameAnti-PKN antibody [EPR3238]
See all PKN primary antibodies
DescriptionRabbit monoclonal [EPR3238] to PKN
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PKN aa 900-1000 (C terminal). The exact sequence is proprietary.
- SH-SY-5Y, U937 and Jurkat cell lysates
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevancePKN belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis.
- DBK antibody
- DPKN antibody
- PAK 1 antibody
All lanes : Anti-PKN antibody [EPR3238] (ab108973) at 1/1000 dilution
Lane 1 : SH-SY-5Y cell lysates
Lane 2 : U937 cell lysates
Lane 3 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 104 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Flow cytometric analysis of PKN in permeabilized Jurkat cells, using a 1/10 dilution of ab108973 (red) or a rabbit IgG (negative) (green).
ab108973 has not yet been referenced specifically in any publications.