Recombinant
RabMAb

Recombinant Anti-PKN antibody [EPR3238] - BSA and Azide free (ab247726)

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Overview

  • Product name

    Anti-PKN antibody [EPR3238] - BSA and Azide free
    See all PKN primary antibodies

  • Description

    Rabbit monoclonal [EPR3238] to PKN - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PKN aa 900-1000 (C terminal). The exact sequence is proprietary.

  • General notes

    Ab247726 is the carrier-free version of ab108973. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247726 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3238

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab247726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa).
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Relevance

      PKN belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis.

    • Cellular localization

      Cytoplasmic

    • Database links

      see all

    • Alternative names

      • DBK antibody
      • DPKN antibody
      • PAK 1 antibody
      • PRK 1 antibody
      • PROTEIN KINASE C RELATED KINASE 1 antibody
      • SERINE/THREONINE PROTEIN KINASE N antibody
      see all

    References

    ab247726 has not yet been referenced specifically in any publications.

    Publishing research using ab247726? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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