Overview

  • Product name

  • Description

    Rabbit polyclonal to PKN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human PKN1 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: Q16512

  • Positive control

    • IHC-P: Human colon and kidney tissues. ICC/IF: HepG2 cells. WB: HEK-293T, HepG2, SP2/0 and NIH/3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab231038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/1000. Predicted molecular weight: 104 kDa.

Target

  • Function

    PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.
  • Tissue specificity

    Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 1 protein kinase domain.
    Contains 3 REM (Hr1) repeats.
  • Domain

    The C1 domain does not bind the diacylglycerol (DAG).
  • Post-translational
    modifications

    Autophosphorylated; preferably on serine. Phosphorylated during mitosis.
    Activated by limited proteolysis with trypsin.
  • Cellular localization

    Cytoplasm. Nucleus. Endosome. Cell membrane. Cleavage furrow. Midbody. Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-374 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis.
  • Information by UniProt
  • Database links

  • Alternative names

    • DBK antibody
    • PAK 1 antibody
    • PAK-1 antibody
    • PAK1 antibody
    • PKC1 antibody
    • PKN ALPHA antibody
    • PKN antibody
    • Pkn1 antibody
    • PKN1_HUMAN antibody
    • PRK1 antibody
    • PRKCL1 antibody
    • Protease activated kinase 1 antibody
    • Protease-activated kinase 1 antibody
    • Protein kinase C like 1 antibody
    • Protein kinase C like PKN antibody
    • Protein kinase C related kinase 1 antibody
    • Protein kinase C-like 1 antibody
    • Protein kinase C-like PKN antibody
    • Protein kinase N1 antibody
    • Protein kinase PKN alpha antibody
    • Protein kinase PKN-alpha antibody
    • Protein-kinase C-related kinase 1 antibody
    • Serine threonine kinase N antibody
    • Serine threonine protein kinase N antibody
    • Serine-threonine protein kinase N antibody
    • Serine/threonine protein kinase N1 antibody
    • Serine/threonine-protein kinase N1 antibody
    see all

Images

  • All lanes : Anti-PKN1 antibody (ab231038) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : SP2/0 whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Predicted band size: 104 kDa

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for PKN1 (red) using ab231038 at 1/100 dilution in ICC/IF.

    Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for PKN1 using ab231038 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for PKN1 using ab231038 at 1/100 dilution in immunohistochemical analysis.

References

ab231038 has not yet been referenced specifically in any publications.

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