Recombinant Anti-PKN1 antibody [EPR18808] (ab195264)
![Western blot - Anti-PKN1 antibody [EPR18808] (ab195264)](https://www.abcam.com/ps/products/195/ab195264/Images/ab195264-268422-anti-pkn1-antibody-epr18808-western-blot.jpg "Western blot - Anti-PKN1 antibody [EPR18808] (ab195264)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18808] to PKN1
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PKN1 antibody [EPR18808]
See all PKN1 primary antibodies -
Description
Rabbit monoclonal [EPR18808] to PKN1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, LNCaP, MDA-MB-231, K562, HEK-293, MCF7, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Rat brain and spleen lysates. IP: Jurkat whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18808 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/40.
|
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| WB | (1) |
1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa).
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| Notes |
|---|
|
IP
1/40. |
|
WB
1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa). |
Target
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Function
PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro. -
Tissue specificity
Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 1 protein kinase domain.
Contains 3 REM (Hr1) repeats. -
Domain
The C1 domain does not bind the diacylglycerol (DAG). -
Post-translational
modificationsAutophosphorylated; preferably on serine. Phosphorylated during mitosis.
Activated by limited proteolysis with trypsin. -
Cellular localization
Cytoplasm. Nucleus. Endosome. Cell membrane. Cleavage furrow. Midbody. Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-374 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. - Information by UniProt
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Database links
- Entrez Gene: 5585 Human
- Entrez Gene: 320795 Mouse
- Entrez Gene: 29355 Rat
- Omim: 601032 Human
- SwissProt: Q16512 Human
- SwissProt: P70268 Mouse
- SwissProt: Q63433 Rat
- Unigene: 466044 Human
see all -
Alternative names
- DBK antibody
- PAK 1 antibody
- PAK-1 antibody
see all
Images
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Western blot - Anti-PKN1 antibody [EPR18808] (ab195264)
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: PKN1 knockout HAP1 cell lysate (40 µg)
Lane 3: MCF7 cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab195264 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.ab195264 was shown to specifically react with PKN1 when PKN1 knockout samples were used. Wild-type and PKN1 knockout samples were subjected to SDS-PAGE. Ab195264 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PKN1 antibody [EPR18808] (ab195264)](https://www.abcam.com/ps/products/195/ab195264/Images/ab195264-256458-anti-pkn1-antibody-epr18808-western-blot.jpg)
Western blot - Anti-PKN1 antibody [EPR18808] (ab195264)All lanes : Anti-PKN1 antibody [EPR18808] (ab195264) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : LNCaP (Human prostate cancer cell line) whole cell lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 104 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-PKN1 antibody [EPR18808] (ab195264)](https://www.abcam.com/ps/products/195/ab195264/Images/ab195264-256452-anti-pkn1-antibody-epr18808-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PKN1 antibody [EPR18808] (ab195264)PKN1 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with ab195264 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab195264 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Jurkat whole cell lysate, 10µg (Input).
Lane 2: ab195264 IP in Jurkat whole cell lysate.
Lane 3: Rabbit IgG,monoclonal-Isotype Control (ab172730) instead of ab195264 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Western blot - Anti-PKN1 antibody [EPR18808] (ab195264)](https://www.abcam.com/ps/products/195/ab195264/Images/ab195264-256455-anti-pkn1-antibody-epr18808-western-blot.jpg)
Western blot - Anti-PKN1 antibody [EPR18808] (ab195264)All lanes : Anti-PKN1 antibody [EPR18808] (ab195264) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 104 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-PKN1 antibody [EPR18808] (ab195264)](https://www.abcam.com/ps/products/195/ab195264/Images/ab195264-256453-anti-pkn1-antibody-epr18808-western-blot.jpg)
Western blot - Anti-PKN1 antibody [EPR18808] (ab195264)All lanes : Anti-PKN1 antibody [EPR18808] (ab195264) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 104 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
![Anti-PKN1 antibody [EPR18808] (ab195264)](https://www.abcam.com/ps/products/195/ab195264/Images/ab195264-6-benefits-of-recombinant-antibodies.png)
Anti-PKN1 antibody [EPR18808] (ab195264)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (2)
ab195264 has been referenced in 2 publications.
- Patel H et al. Novel roles of PRK1 and PRK2 in cilia and cancer biology. Sci Rep 10:3902 (2020). PubMed: 32127582
- Chang J et al. Exome-wide analysis identifies three low-frequency missense variants associated with pancreatic cancer risk in Chinese populations. Nat Commun 9:3688 (2018). PubMed: 30206226
