Anti-PKN2 antibody (ab87812)
Key features and details
- Rabbit polyclonal to PKN2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PKN2 antibody
See all PKN2 primary antibodies -
Description
Rabbit polyclonal to PKN2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide corresponding to a region between residue 200 and 250 of human PKN2 (NP_006247.1).
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Positive control
- Whole cell lysates from HeLa and 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab87812 was affinity purified using an epitope specific to PKN2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 112 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 112 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Exhibits a preference for highly basic protein substrates. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 1 protein kinase domain.
Contains 3 REM (Hr1) repeats. -
Domain
The C1 domain does not bind the diacylglycerol (DAG). -
Post-translational
modificationsAutophosphorylated.
Activated by limited proteolysis with trypsin. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 424519 Chicken
- Entrez Gene: 5586 Human
- Entrez Gene: 109333 Mouse
- Entrez Gene: 207122 Rat
- Omim: 602549 Human
- SwissProt: Q16513 Human
- SwissProt: Q8BWW9 Mouse
- SwissProt: O08874 Rat
see all -
Alternative names
- Cardiolipin activated protein kinase Pak2 antibody
- MGC150606 antibody
- MGC71074 antibody
see all
Images
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All lanes : Anti-PKN2 antibody (ab87812) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 112 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
Detection of PKN2 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87812 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab87812 has not yet been referenced specifically in any publications.