Key features and details
- Rabbit polyclonal to PKN2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PKN2 antibody
See all PKN2 primary antibodies
DescriptionRabbit polyclonal to PKN2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to a region between residue 200 and 250 of human PKN2 (NP_006247.1).
- Whole cell lysates from HeLa and 293T cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab87812 was affinity purified using an epitope specific to PKN2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab87812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 112 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionExhibits a preference for highly basic protein substrates.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 1 protein kinase domain.
Contains 3 REM (Hr1) repeats.
DomainThe C1 domain does not bind the diacylglycerol (DAG).
Activated by limited proteolysis with trypsin.
- Information by UniProt
- Cardiolipin activated protein kinase Pak2 antibody
- MGC150606 antibody
- MGC71074 antibody
All lanes : Anti-PKN2 antibody (ab87812) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 112 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
Detection of PKN2 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87812 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab87812 has not yet been referenced specifically in any publications.