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Rabbit polyclonal to PKN2
Predicted to work with:
Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to a region between residue 200 and 250 of human PKN2 (NP_006247.1).
Whole cell lysates from HeLa and 293T cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab87812 was affinity purified using an epitope specific to PKN2 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2 - 5 µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 112 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Exhibits a preference for highly basic protein substrates.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 1 protein kinase domain. Contains 3 REM (Hr1) repeats.
The C1 domain does not bind the diacylglycerol (DAG).
Activated by limited proteolysis with trypsin.
Information by UniProt
Cardiolipin activated protein kinase Pak2 antibody
Western blot - Anti-PKN2 antibody (ab87812)
All lanes :
Anti-PKN2 antibody (ab87812) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size:
Observed band size:
117 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-PKN2 antibody (ab87812)
Detection of PKN2 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87812 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"