Overview

  • Product name

    Anti-PKN2 antibody [EPR5490]
    See all PKN2 primary antibodies
  • Description

    Rabbit monoclonal [EPR5490] to PKN2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PKN2 aa 900-1000. The exact sequence is proprietary.

  • Positive control

    • 293T, Jurkat, HeLa, and HepG2 whole cell lysate (ab7900), HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5490
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Exhibits a preference for highly basic protein substrates.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 1 protein kinase domain.
      Contains 3 REM (Hr1) repeats.
    • Domain

      The C1 domain does not bind the diacylglycerol (DAG).
    • Post-translational
      modifications

      Autophosphorylated.
      Activated by limited proteolysis with trypsin.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cardiolipin activated protein kinase Pak2 antibody
      • MGC150606 antibody
      • MGC71074 antibody
      • PAK 2 antibody
      • PAK2 antibody
      • PKN gamma antibody
      • PKN2 antibody
      • PKN2_HUMAN antibody
      • PRK2 antibody
      • PRKCL2 antibody
      • PRO2042 antibody
      • Protein kinase C like 2 antibody
      • Protein kinase C related kinase 2 antibody
      • Protein kinase C-like 2 antibody
      • Protein kinase N2 antibody
      • Protein-kinase C-related kinase 2 antibody
      • Serine/threonine-protein kinase N2 antibody
      see all

    Images

    • All lanes : Anti-PKN2 antibody [EPR5490] (ab138514) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 112 kDa
      Observed band size: 112 kDa

    • Immunofluorescent analysis of HeLa cells labelling PKN2 with ab138514 at 1/100 dilution.

    References

    This product has been referenced in:

    • Guen VJ  et al. STAR syndrome-associated CDK10/Cyclin M regulates actin network architecture and ciliogenesis. Cell Cycle 15:678-88 (2016). Read more (PubMed: 27104747) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab138514.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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