Recombinant Anti-PKN2 antibody [EPR5490] - BSA and Azide free (ab248857)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5490] to PKN2 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PKN2 antibody [EPR5490] - BSA and Azide free
See all PKN2 primary antibodies -
Description
Rabbit monoclonal [EPR5490] to PKN2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HCT116, HEK-293T, Jurkat, HeLa, and HepG2 (ab7900) cell lysates. ICC/IF: HeLa cells.
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General notes
ab248857 is the carrier-free version of ab138514.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR5490 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa). |
Target
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Function
Exhibits a preference for highly basic protein substrates. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 1 protein kinase domain.
Contains 3 REM (Hr1) repeats. -
Domain
The C1 domain does not bind the diacylglycerol (DAG). -
Post-translational
modificationsAutophosphorylated.
Activated by limited proteolysis with trypsin. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5586 Human
- Entrez Gene: 109333 Mouse
- Entrez Gene: 207122 Rat
- Omim: 602549 Human
- SwissProt: Q16513 Human
- SwissProt: Q8BWW9 Mouse
- SwissProt: O08874 Rat
- Unigene: 440833 Human
see all -
Alternative names
- Cardiolipin activated protein kinase Pak2 antibody
- MGC150606 antibody
- MGC71074 antibody
see all
Images
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All lanes : Anti-PKN2 antibody [EPR5490] (ab138514) at 1/1000 dilution
Lane 1 : Wild-type HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 2 : PKN2 knockout HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 112 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?This data was developed using ab138514, the same antibody clone in a different buffer formulation.
Lanes 1-3: Merged signal (red and green). Green - ab138514 observed at 125 kDa. Red - loading control ab8245 observed at 36 kDa.
ab138514 Anti-PKN2 antibody [EPR5490] (ab138514) was shown to specifically react with PKN2 in wild-type HCT cells. Loss of signal was observed when knockout cell line ab266894 (knockout cell lysate ab258588) was used. Wild-type and PKN2 knockout samples were subjected to SDS-PAGE. ab138514 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-PKN2 antibody [EPR5490] (ab138514) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PKN2 knockout HeLa cell lysate
Lane 3 : HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 112 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab138514).
Lanes 1-3: Merged signal (red and green). Green - ab138514 observed at 112 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab138514 Anti-PKN2 antibody [EPR5490] was shown to specifically react with PKN2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264691 (knockout cell lysate ab258587) was used. Wild-type and PKN2 knockout samples were subjected to SDS-PAGE. ab138514 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248857 has not yet been referenced specifically in any publications.