Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3511(2)] to PKN3
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-PKN3 antibody [EPR3511(2)]
See all PKN3 primary antibodies
DescriptionRabbit monoclonal [EPR3511(2)] to PKN3
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PKN3 aa 100-200. The exact sequence is proprietary.
Database link: Q6P5Z2
- WB: PC-3, Mouse spleen, Rat spleen lysates ICC/IF: LNCaP cells. Flow Cyt: LNCaP cells.
This product was previously labelled as PKN beta
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab155076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 99 kDa).|
For unpurified use at 1/100 - 1/250.
FunctionContributes to invasiveness in malignant prostate cancer.
Tissue specificityExpressed in prostate tumors and various cancer cell lines. Not expressed in adult tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 3 REM (Hr1) repeats.
DomainThe C1 domain does not bind the diacylglycerol (DAG).
Cellular localizationNucleus. Cytoplasm > perinuclear region. Nuclear and perinuclear Golgi region.
- Information by UniProt
- pkn3 antibody
- PKN3_HUMAN antibody
- PKNBETA antibody
Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling PKN3 with Purified ab155076 at 1:300 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling PKN3 with Purified ab155076 at 1:300 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
All lanes : Anti-PKN3 antibody [EPR3511(2)] (ab155076) at 1/5000 dilution (Purified)
Lane 1 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse spleen lysates
Lane 3 : Rat spleen lysates
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
All lanes : Anti-PKN3 antibody [EPR3511(2)] (ab155076) at 1/1000 dilution
Lane 1 : LNCaP cell lysate
Lane 2 : PC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 99 kDa
This image was generated using the unpurified version of the product.
ab155076 has been referenced in 1 publication.
- Uehara S et al. Protein kinase N3 promotes bone resorption by osteoclasts in response to Wnt5a-Ror2 signaling. Sci Signal 10:N/A (2017). PubMed: 28851822