Overview

  • Product name

  • Description

    Rabbit polyclonal to PKR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment within Human PKR (internal sequence). The exact sequence is proprietary.
    Database link: P19525

  • Positive control

    • WB: MCF 7 and MDA-MB-231 cell lysate. IP: MCF7 cell lysate. IHC: Human lung cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 62 kDa.
IP 1/100 - 1/521.

Target

  • Function

    Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
  • Information by UniProt
  • Database links

  • Alternative names

    • Double stranded RNA activated protein kinase; antibody
    • E2AK2_HUMAN antibody
    • eIF-2A protein kinase 2 antibody
    • EIF2AK1 antibody
    • EIF2AK2 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 2 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 2 antibody
    • HGNC:9437 antibody
    • Interferon induced double stranded RNA activated protein kinase antibody
    • Interferon inducible elF2 alpha kinase antibody
    • Interferon inducible RNA dependent protein kinase antibody
    • Interferon-induced, double-stranded RNA-activated protein kinase antibody
    • Interferon-inducible RNA-dependent protein kinase antibody
    • MGC126524 antibody
    • P1/eIF-2A protein kinase antibody
    • P1/eIF2A protein kinase antibody
    • p68 kinase antibody
    • PKR antibody
    • PPP1R83 antibody
    • PRKR antibody
    • Protein kinase interferon inducible double stranded RNA dependent antibody
    • Protein kinase RNA activated antibody
    • Protein kinase RNA-activated antibody
    • Protein phosphatase 1 regulatory subunit 83 antibody
    • Serine/threonine protein kinase TIK antibody
    • Tyrosine protein kinase EIF2AK2 antibody
    see all

Images

  • All lanes : Anti-PKR antibody (ab226819) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 62 kDa

  • PKR was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell lysate with ab226819. Western blot was performed from the immunoprecipitate using ab226819.

    Lane 1: Rabbit IgG instead of ab226819 in MCF7 whole cell lysate.
    Lane 2: ab226819 IP in MCF7 whole cell lysate.

  • Paraffin embedded human lung cancer tissue stained for PKR using ab226819 at 1/500 dilution in immunohistochemical analysis.

References

ab226819 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226819.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up