Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
20 µg
Specification
HEK293
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit 680LT IRDye (Odyssey)
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 680LT
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 680LT
Dilution
1/10000
Detection
Detection method
Li-Cor
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 68 kDa
Positive control
First lane - Bio-Rad Precision Plus ladder (75 kDa to 250 kDa shown)
Second lane - Total cell lysate
Second lane - Total cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Samantha Johnston
Verified customer
Submitted Sep 27 2011