Key features and details
- Rabbit polyclonal to PKR (phospho T446)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PKR (phospho T446) antibody
See all PKR primary antibodies
DescriptionRabbit polyclonal to PKR (phospho T446)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide within Human PKR (internal sequence) (phospho T446). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: P19525
- WB: IFN gamma treated HeLa whole cell extract.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226852 in the following tested applications.
|WB||1/500 - 1/3000. Predicted molecular weight: 62 kDa.|
FunctionFollowing activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 protein kinase domain.
modificationsAutophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
- Information by UniProt
- Double stranded RNA activated protein kinase; antibody
- E2AK2_HUMAN antibody
- eIF-2A protein kinase 2 antibody
All lanes : Anti-PKR (phospho T446) antibody (ab226852) at 1/500 dilution
Lane 1 : IFN gamma (10 ng/ml, 24 hours) treated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : IFN gamma (10 ng/ml, 24 hours), Calyculin A (50 nM, 30 min) treated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 60 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 62 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226852 has not yet been referenced specifically in any publications.