Anti-PKR (phospho T446) antibody [E120] (ab32036)
- Datasheet
- Specific References (50)
- Protocols
Overview
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Product nameAnti-PKR (phospho T446) antibody [E120]
See all PKR primary antibodies -
DescriptionRabbit monoclonal [E120] to PKR (phospho T446)
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Host speciesRabbit
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SpecificityThe antibody only detects PKR phosphorylated on Threonine 446.
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Tested applicationsSuitable for: ChIP, IHC-P, WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human PKR. The exact sequence is proprietary.
(Peptide available asab181660) -
Positive control
- Calynculin A and TNF alpha treated HeLa cell lysate
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberE120
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab32036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ChIP | Use a concentration of 4 µg/ml. | |
| IHC-P | 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).Can be blocked with PKR (phospho T446) peptide (ab181660). | |
| IP | 1/10. |
Target
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FunctionFollowing activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
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Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase. - Information by UniProt
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Database links
- Entrez Gene: 5610 Human
- Entrez Gene: 19106 Mouse
- Omim: 176871 Human
- SwissProt: P19525 Human
- SwissProt: Q03963 Mouse
- Unigene: 131431 Human
- Unigene: 378990 Mouse
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Alternative names
- Double stranded RNA activated protein kinase; antibody
- E2AK2_HUMAN antibody
- eIF-2A protein kinase 2 antibody
see all
Images
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![Western blot - Anti-PKR (phospho T446) antibody [E120] (ab32036)](https://www.abcam.com/ps/products/32/ab32036/Images/ab32036-247703-32036.jpg)
Western blot - Anti-PKR (phospho T446) antibody [E120] (ab32036)All lanes : Anti-PKR (phospho T446) antibody [E120] (ab32036) at 1/1000 dilution (purified)
Lane 1 : HeLa (human cervix adenocarcinoma) treated with Calyculin A and TNF-alpha whole cell lysates
Lane 2 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 62 kDa
Observed band size: 68 kDa (why is the actual band size different from the predicted?)Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-PKR (phospho T446) antibody [E120] (ab32036)](https://www.abcam.com/ps/products/32/ab32036/Images/ab32036wb.jpg)
Western blot - Anti-PKR (phospho T446) antibody [E120] (ab32036)All lanes : Anti-PKR (phospho T446) antibody [E120] (ab32036) at 1/1000 dilution (unpurified)
Lane 1 : Hela cell lysate
Lane 2 : Hela cell lysate + IFN + CalyculinA
Lane 3 : Hela cell lysate + Pptase
Predicted band size: 62 kDa
Observed band size: 68 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKR (phospho T446) antibody [E120] (ab32036)](https://www.abcam.com/ps/products/32/ab32036/Images/ab32036ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKR (phospho T446) antibody [E120] (ab32036)Immunohistochemical analysis of PKR (phospho T446) expression in normal colon tissue, using 1/50 unpurified ab32036.
References
This product has been referenced in:
- Krishnamoorthy J et al. Downregulation of PERK activity and eIF2a serine 51 phosphorylation by mTOR complex 1 elicits pro-oxidant and pro-death effects in tuberous sclerosis-deficient cells. Cell Death Dis 9:254 (2018). WB . Read more (PubMed: 29449538) »
- Pan X et al. Heat shock factor 1 suppresses the HIV-induced inflammatory response by inhibiting nuclear factor-?B. Cell Immunol 327:26-35 (2018). Read more (PubMed: 29525181) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"