Recombinant Anti-PKR (phospho T446) antibody [E120] - BSA and Azide free (ab220801)
![Western blot - Anti-PKR (phospho T446) antibody [E120] - BSA and Azide free (ab220801)](https://www.abcam.com/ps/products/220/ab220801/Images/ab220801-395135-anti-pkr-phospho-t446-antibody-e120-western-blot-calyculin-a-tnf-alpha-treated-hela.jpg "Western blot - Anti-PKR (phospho T446) antibody [E120] - BSA and Azide free (ab220801)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E120] to PKR (phospho T446) - BSA and Azide free
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-PKR (phospho T446) antibody [E120] - BSA and Azide free
See all PKR primary antibodies -
Description
Rabbit monoclonal [E120] to PKR (phospho T446) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. The exact sequence is proprietary.
(Peptide available asab181660) -
Positive control
- WB: HeLa treated with Calyculin A and TNF-alpha whole cell lysate; Calynculin A and IFN treated HeLa cell lysate. IHC-P: Human colon tissue.
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General notes
ab220801 is the carrier-free version of ab32036 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab220801 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E120 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab220801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa). | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase. - Information by UniProt
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Database links
- Entrez Gene: 5610 Human
- Omim: 176871 Human
- SwissProt: P19525 Human
- Unigene: 131431 Human
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Alternative names
- Double stranded RNA activated protein kinase; antibody
- E2AK2_HUMAN antibody
- eIF-2A protein kinase 2 antibody
see all
Images
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Western blot - Anti-PKR (phospho T446) antibody [E120] - BSA and Azide free (ab220801)All lanes : Anti-PKR (phospho T446) antibody [E120] (ab32036) at 1/2000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma) treated with 100 ng/mL Calyculin A whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma) treated with 100 ng/mL Calyculin A followed by 20 ng/mL TNF alpha whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Diluting buffer: 5% NFDM/TBST
Positive control: Rabbit monoclonal [Y117] to PKR (ab32506).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32036).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKR (phospho T446) antibody [E120] - BSA and Azide free (ab220801)](https://www.abcam.com/ps/products/220/ab220801/Images/ab220801-329267-anti-pkr-phospho-t446-antibody-e120-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKR (phospho T446) antibody [E120] - BSA and Azide free (ab220801)Immunohistochemical analysis of PKR (phospho T446) expression in normal colon tissue, using 1/50 unpurified ab32036.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32036).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-PKR (phospho T446) antibody [E120] - BSA and Azide free (ab220801)](https://www.abcam.com/ps/products/220/ab220801/Images/ab220801-3-benefits-of-recombinant-antibodies.png)
Anti-PKR (phospho T446) antibody [E120] - BSA and Azide free (ab220801)
Protocols
Datasheets and documents
Certificate of Compliance
References (26)
ab220801 has been referenced in 26 publications.
- Kainulainen M et al. NSs Virulence Factor of Rift Valley Fever Virus Engages the F-Box Proteins FBXW11 and ß-TRCP1 To Degrade the Antiviral Protein Kinase PKR. J Virol 90:6140-7 (2016). PubMed: 27122577
- Rabouw HH et al. Middle East Respiratory Coronavirus Accessory Protein 4a Inhibits PKR-Mediated Antiviral Stress Responses. PLoS Pathog 12:e1005982 (2016). WB ; Human . PubMed: 27783669
- Pfaller CK et al. Measles virus C protein impairs production of defective copyback double-stranded viral RNA and activation of protein kinase R. J Virol 88:456-68 (2014). WB . PubMed: 24155404
- Nejepinska J et al. Reporters transiently transfected into mammalian cells are highly sensitive to translational repression induced by dsRNA expression. PLoS One 9:e87517 (2014). WB ; Human . PubMed: 24475301
- Wen X et al. NF90 exerts antiviral activity through regulation of PKR phosphorylation and stress granules in infected cells. J Immunol 192:3753-64 (2014). WB . PubMed: 24623135
