Key features and details
- Rabbit polyclonal to PLA1A/NMD
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-PLA1A/NMD antibody
See all PLA1A/NMD primary antibodies
DescriptionRabbit polyclonal to PLA1A/NMD
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Orangutan
Recombinant fragment (His-tag) corresponding to Human PLA1A/NMD aa 309-452. (Expressed in E.coli).
LVEQGGVKIEPLPKEVKVYLLTTSSAPYCMHHSLVEFHLKELRNKDTNIE VTFLSSNITSSSKITIPKQQRYGKGIIAHATPQCQINQVKFKFQSSNRVW KKDRTTIIGKFCTALLPVNDREKMVCLPEPVNLQASVTVSCDLK
Database link: Q53H76
- WB: HEK-293T cell lysate; Pig liver lysate; Recombinant human PLA1A/NMD protein. IHC-P: Human lung and kidney tissues.
This product was previously labelled as PLA1A
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab233145 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 50 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionHydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1-acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Isoform 2 hydrolyzes lyso-PS but not PS. Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production.
Tissue specificityWidely expressed. Expressed in placenta, prostate and liver. Weakly or not expressed in skin, leukocytes, platelets, colon, spleen, lung, muscle and kidney.
Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family.
- Information by UniProt
- AA986889 antibody
- NMD antibody
- OTTHUMP00000215248 antibody
Formalin-fixed, paraffin-embedded human lung tissue stained for PLA1A/NMD using ab233145 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human kidney tissue stained for PLA1A/NMD using ab233145 at 20 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-PLA1A/NMD antibody (ab233145) at 1.5 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Pig liver lysate
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/1000 dilution
Predicted band size: 50 kDa
Anti-PLA1A/NMD antibody (ab233145) at 2 µg/ml + Recombinant human PLA1A/NMD protein
Predicted band size: 50 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233145 has not yet been referenced specifically in any publications.