• Product name

  • Description

    Rabbit polyclonal to PLA2G3
  • Host species

  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PLA2G3 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control

    • Human colonic carcinoma tissue



Our Abpromise guarantee covers the use of ab62197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 µg/ml.


  • Function

    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Shows an 11-fold preference for phosphatidylglycerol over phosphatidylcholine (PC). Preferential cleavage: 1-palmitoyl-2-linoleoyl-phosphatidylethanolamine (PE) > 1-palmitoyl-2-linoleoyl-PC > 1-palmitoyl-2-arachidonoyl-PC > 1-palmitoyl-2-arachidonoyl-PE. Plays a role in ciliogenesis.
  • Tissue specificity

    Expressed in kidney, heart, liver, and skeletal muscle. Also present in placenta and peripheral blood leukocytes. Not detected in brain, colon, thymus, spleen and small intestine. In lung, expressed in bronchial epithelial cells and alveolar macrophages, but scarcely detected in alveolar epithelium, arterial walls and interstitial fibroblasts (at protein level). In joints of osteoarthritis and rheumatoid arthritis, expressed in endothelial cells (at protein level). In normal heart, detected in some vessels. In myocardial tissues with acute infarction, expressed in vascular endothelial cells adjacent to cardiomyocytes and those in lesions with granulation. Expression in cardiomyocytes is scarce (at protein level). In uterus, breast and colon cancers, detected in tumor cells and neighboring microvascular endothelium, but not in normal glandular tissues (at protein level).
  • Sequence similarities

    Belongs to the phospholipase A2 family.
  • Post-translational

    N-glycosylation does not affect the catalytic activity, but is required for proper secretion. A nonglycosylated form was observed in several cell types.
    In several cell types, the N- and C-termini are cleaved off.
  • Cellular localization

    Secreted. Cell membrane. Cytoplasm > cytoskeleton > centrosome > centriole.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC antibody
    • GIII sPLA2 antibody
    • Group 3 secretory phospholipase A2 antibody
    • Group III secretory phospholipase A2 antibody
    • PA2G3_HUMAN antibody
    • Phosphatidylcholine 2 acylhydrolase GIII antibody
    • Phosphatidylcholine 2-acylhydrolase 3 antibody
    • phospholipase A2 group III antibody
    • PLA2G3 antibody
    • sPLA2-III antibody
    • SPLA2III antibody
    see all


  • Immunohistochemical analysis of paraffin embedded colonic carcinoma tissue, using ab62197 at 2.5µg/ml.


ab62197 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you for your response.
Currently, we can't offer customer made peptide synthesis. It is very likely that we currently we do not have specific antibodies which could target the central S domain of the PLA2g3 protein (149-291 of the human PLA2G3 protein sequence) as your customer. Unfortunately, the immunogen/blocking peptides are not available commercially.
Is there any specific reason why the customer would be interested in targeting this region?
If you need anything further or any help then please let me know.

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Thank you for your enquiry.
These two antibodies are from two different sources, the manufacturing and the characterization processes are different thus the tested applications are not the same as you can see of the product datasheets.
I can release that the immunogen used to raise ab96533 was a recombinant fragment corresponding to a region within amino acids 263-472 of Human PLA2G3 (NP_056530). Unfortunately, the blocking peptide is not available commercially.
If you need any further assistance in the future, please do not hesitate to contact me.

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Thank you for your inquiry.
The exact sequence used to generate this antibody is considered proprietary but I can release that it is taken from within the general range of aa 160-210 of the human protein according to NP_056530.2, so it is within aa 149-291.
This antibody has been evaluated only for use in IHC-P with human tissues fixed in 10% neutral buffered formalin and embedded in paraffin. No additional testing has been performed at this time.
The antibody you mentioned is a polyclonal antibody. Polyclonal antibodies consist of a mixed population of IgGs each of which will recognize a different epitope. Generally, epitope mapping is not carried out for polyclonal antibodies.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

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