Key features and details
- Rabbit polyclonal to PLA2G3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PLA2G3 antibody
See all PLA2G3 primary antibodies
DescriptionRabbit polyclonal to PLA2G3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to a region within amino acids 263-472 of Human PLA2G3 (NP_056530).
- MOLT4 whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 57 kDa.|
FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Shows an 11-fold preference for phosphatidylglycerol over phosphatidylcholine (PC). Preferential cleavage: 1-palmitoyl-2-linoleoyl-phosphatidylethanolamine (PE) > 1-palmitoyl-2-linoleoyl-PC > 1-palmitoyl-2-arachidonoyl-PC > 1-palmitoyl-2-arachidonoyl-PE. Plays a role in ciliogenesis.
Tissue specificityExpressed in kidney, heart, liver, and skeletal muscle. Also present in placenta and peripheral blood leukocytes. Not detected in brain, colon, thymus, spleen and small intestine. In lung, expressed in bronchial epithelial cells and alveolar macrophages, but scarcely detected in alveolar epithelium, arterial walls and interstitial fibroblasts (at protein level). In joints of osteoarthritis and rheumatoid arthritis, expressed in endothelial cells (at protein level). In normal heart, detected in some vessels. In myocardial tissues with acute infarction, expressed in vascular endothelial cells adjacent to cardiomyocytes and those in lesions with granulation. Expression in cardiomyocytes is scarce (at protein level). In uterus, breast and colon cancers, detected in tumor cells and neighboring microvascular endothelium, but not in normal glandular tissues (at protein level).
Sequence similaritiesBelongs to the phospholipase A2 family.
modificationsN-glycosylation does not affect the catalytic activity, but is required for proper secretion. A nonglycosylated form was observed in several cell types.
In several cell types, the N- and C-termini are cleaved off.
Cellular localizationSecreted. Cell membrane. Cytoplasm > cytoskeleton > centrosome > centriole.
- Information by UniProt
- EC 126.96.36.199 antibody
- GIII sPLA2 antibody
- Group 3 secretory phospholipase A2 antibody
ab96533 has been referenced in 1 publication.
- Roy D et al. Loss of HSulf-1 promotes altered lipid metabolism in ovarian cancer. Cancer Metab 2:13 (2014). WB . PubMed: 25225614