Overview

  • Product name

  • Description

    Rabbit polyclonal to PLA2G3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 263-472 of Human PLA2G3 (NP_056530).

  • Positive control

    • MOLT4 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab96533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.

Target

  • Function

    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Shows an 11-fold preference for phosphatidylglycerol over phosphatidylcholine (PC). Preferential cleavage: 1-palmitoyl-2-linoleoyl-phosphatidylethanolamine (PE) > 1-palmitoyl-2-linoleoyl-PC > 1-palmitoyl-2-arachidonoyl-PC > 1-palmitoyl-2-arachidonoyl-PE. Plays a role in ciliogenesis.
  • Tissue specificity

    Expressed in kidney, heart, liver, and skeletal muscle. Also present in placenta and peripheral blood leukocytes. Not detected in brain, colon, thymus, spleen and small intestine. In lung, expressed in bronchial epithelial cells and alveolar macrophages, but scarcely detected in alveolar epithelium, arterial walls and interstitial fibroblasts (at protein level). In joints of osteoarthritis and rheumatoid arthritis, expressed in endothelial cells (at protein level). In normal heart, detected in some vessels. In myocardial tissues with acute infarction, expressed in vascular endothelial cells adjacent to cardiomyocytes and those in lesions with granulation. Expression in cardiomyocytes is scarce (at protein level). In uterus, breast and colon cancers, detected in tumor cells and neighboring microvascular endothelium, but not in normal glandular tissues (at protein level).
  • Sequence similarities

    Belongs to the phospholipase A2 family.
  • Post-translational
    modifications

    N-glycosylation does not affect the catalytic activity, but is required for proper secretion. A nonglycosylated form was observed in several cell types.
    In several cell types, the N- and C-termini are cleaved off.
  • Cellular localization

    Secreted. Cell membrane. Cytoplasm > cytoskeleton > centrosome > centriole.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 3.1.1.4 antibody
    • GIII sPLA2 antibody
    • Group 3 secretory phospholipase A2 antibody
    • Group III secretory phospholipase A2 antibody
    • PA2G3_HUMAN antibody
    • Phosphatidylcholine 2 acylhydrolase GIII antibody
    • Phosphatidylcholine 2-acylhydrolase 3 antibody
    • phospholipase A2 group III antibody
    • PLA2G3 antibody
    • sPLA2-III antibody
    • SPLA2III antibody
    see all

Images

  • Anti-PLA2G3 antibody (ab96533) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your response.
Currently, we can't offer customer made peptide synthesis. It is very likely that we currently we do not have specific antibodies which could target the central S domain of the PLA2g3 protein (149-291 of the human PLA2G3 protein sequence) as your customer. Unfortunately, the immunogen/blocking peptides are not available commercially.
Is there any specific reason why the customer would be interested in targeting this region?
If you need anything further or any help then please let me know.

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Answer

Thank you for your enquiry.
These two antibodies are from two different sources, the manufacturing and the characterization processes are different thus the tested applications are not the same as you can see of the product datasheets.
I can release that the immunogen used to raise ab96533 was a recombinant fragment corresponding to a region within amino acids 263-472 of Human PLA2G3 (NP_056530). Unfortunately, the blocking peptide is not available commercially.
If you need any further assistance in the future, please do not hesitate to contact me.

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