Overview

  • Product name

  • Description

    Rabbit polyclonal to PLA2G5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse PLA2G5 aa 79-94 conjugated to keyhole limpet haemocyanin.
    Sequence:

    CAIRTQSYDY RYTNGL

  • Positive control

    • Expressed in heart, lung, placenta and spleen. IHC-P: FFPE human heart tissue sections.
  • General notes

     This product was previously labelled as Secretory phospholipase A2 Type V

     

Applications

Our Abpromise guarantee covers the use of ab23709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notes
    WB: 1/500. Predicted molecular weight: 15 kDa (Human).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle.
    • Tissue specificity

      Heart, placenta and less abundantly, in lung.
    • Sequence similarities

      Belongs to the phospholipase A2 family.
    • Post-translational
      modifications

      This enzyme lacks one of the seven disulfide bonds found in similar PA2 proteins.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Ca2+ dependent phospholipase A2 antibody
      • Calcium dependent phospholipase A2 antibody
      • Calcium-dependent phospholipase A2 antibody
      • DKFZp686C2294 antibody
      • FRFB antibody
      • Group V phospholipase A2 antibody
      • GV PLA2 antibody
      • gVPLA2 antibody
      • hVPLA(2) antibody
      • MGC46205 antibody
      • OTTHUMP00000044655 antibody
      • PA2G5_HUMAN antibody
      • Phosphatidylcholine 2 acylhydrolase antibody
      • Phosphatidylcholine 2-acylhydrolase 5 antibody
      • Phospholipase A2 group V antibody
      • PLA2 10 antibody
      • PLA2 G5 antibody
      • PLA2-10 antibody
      • Pla2g5 antibody
      • sPLA2 Type V antibody
      see all

    Images

    • IHC image of PLA2G5 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23709, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-PLA2G5 antibody (ab23709) at 1/500 dilution

      Lane 1 : Recombinant human sPLA2 (type V) (100 ng)
      Lane 2 : Recombinant human sPLA2 (type V) (50 ng)
      Lane 3 : Mouse heart homogenate (50ug)

      Predicted band size: 15 kDa
      Observed band size: 15 kDa

    References

    This product has been referenced in:

    • Zhu S  et al. High-Density Lipoprotein (HDL) Counter-Regulates Serum Amyloid A (SAA)-Induced sPLA2-IIE and sPLA2-V Expression in Macrophages. PLoS One 11:e0167468 (2016). WB ; Mouse . Read more (PubMed: 27898742) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab23709.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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